Loss of function of MALAT1 on primary human OA osteoblast transcriptome
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ABSTRACT: Cells: Primary human osteoblasts from subchondral bone of osteoarthritis patients (n=3 patients). Treatments: Cells transfected using Lipofectamine 3000 (Qiagen) with either control LNA (NC) or MALAT1 LNA1 or MALAT1 LNA2 for 24 hours. Total RNA extracted by Qiagen RNeasy columns. RIN values >7 via Agilent Bioanalyser. Analysis: RNA subjected to Quantseq 3' RNA sequencing (Lexogen) and sequenced reads mapped to the hg38 reference human genome.
ORGANISM(S): Homo sapiens
PROVIDER: GSE167918 | GEO | 2021/04/28
REPOSITORIES: GEO
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