Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Spatial transcriptome analysis (Slide-seq2) of mouse main olfactory bulb

ABSTRACT: The goal of spatial transcriptome analysis is to profile the glomeruli position representing different olfactory receptors. 20 OB sections were obtained with equal spacing (130 μm) along the anterior-posterior axis. In total, two sets of 20 slides were obtained from different animals. All of the Slide-seqV2 procedures were performed as previously described

ORGANISM(S): Mus musculus

PROVIDER: GSE169012 | GEO | 2022/01/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Spatial transcriptome analysis (Slide-seq2) of mouse main olfactory bulb

Project description:Spatial transcriptome analysis (Slide-seq2) of mouse main olfactory bulb

| PRJNA714910 | ENA

Rice Transcriptome - Slide A; Slide B

Project description:[Oryza sativa Genome Oligo Set Version 1.0 was designed by Beijing Genomics Institute (BGI) and contain 60,727 70mer oligos representing both indica and japonica genomes. All oligos were designed from cDNAs, expreseed sequence tag (EST) sequences, predicted genes of BGI rice genome build and other public resources. Mapping to TIGR rice genome pseudommolecues release 2 is based on the BLAST results, if a oligo has greater than 97% identity to a gene sequence from TIGR pseudomolecules, the oligo represents that gene. Rice 30K Operon Version 1.0 -70 mer oligos. The array is in 48 pin conformation. It has 25 columns and 25 rows in each of 4 x 12 subarrays. This is the slideA of the two slide sets. Platform_catalog_number: XDEN-A Platform_coating: poly L lysine Platform_manufacture_protocol: Omnigrid 100 contact printer; TeleChem Stealth SMP3 split pins; See http://keck.med.yale.edu/dnaarrays/printing Platform_manufacturer: Keck Biotechnology Resource Lab at Yale Platform_support: glass slide Platform_technology: spotted oligonucleotide ] Series_sample_order: Sample 1-15 Slide A; Sample 16-30 Slide B Keywords: other

2005-05-24 | GSE2691 | GEO

Rice Transcriptome - Slide A; Slide B

2010-06-10 | E-GEOD-2691 | biostudies-arrayexpress

Bulk RNA-sequencing of FACS-sorted kidney glomerular cell types from BTBR ob/ob mice and glomeruli isolated from ex vivo perfused kidneys

Project description:To investigate the gene expression levels of major glomerular cell types in BTBR ob/ob mice and in glomeruli under hyperfiltration Bulk RNA-sequencing of glomerular cell types from BTBR ob/ob and BTBR +/+ mice at three time points (6/12/18 weeks). The glomeruli were isolated from ex vivo perfused kidney (hyperfiltration) and unperfused kidney (control) from the mouse.

2022-11-21 | GSE218086 | GEO

olfactory bulb from control mouse and a mouse with developmentally-induced olfactory bulb degeneration

Project description:The olfactory bulb (OB) is a critical component of mammalian olfactory neuroanatomy. Beyond being the first and sole relay station for olfactory information to the rest of the brain, it also contains elaborate stereotypical circuitry that is considered essential for olfaction. Indeed, substantial lesions of the OB in rodents lead to anosmia. Here, we examined the circuitry that underlies olfaction in a mouse model with severe developmental degeneration of the OB. These mice could perform odor-guided tasks and even responded normally to innate olfactory cues. Despite the near total loss of the OB, piriform cortex in these mice responded to odors normally and its neural activity sufficed to decode odor identity. We found that sensory neurons expressthe full repertoire of olfactory receptors and their axons project primarily to the rudiments of the OB, but also ectopically, to olfactory cortical regions. Within the OB, the number of principal neurons was greatly reduced and the morphology of their dendrites was abnormal, extending over large regions within the OB. Glomerular organization was totally lost in the severe cases of OB degeneration and altered in the more conserved OBs, and the transcriptional profile of the glomerular layer was highly inflammatory. This study shows that olfactory functionality can be preserved despite reduced and aberrant circuitry that is missing many of the elements that are believed to be essential for olfaction, and may explain reported retention of olfaction in humans with degenerated OBs

2023-09-04 | GSE241951 | GEO

Olfactory bulb proteomics in human Alzheimer's disease

Project description:Olfactory dysfunction is among the earliest features of Alzheimer´s disease (AD). Although neuropathological abnormalities have been detected in the olfactory bulb (OB), little is known about its dynamic biology. Here, OB- proteome analysis was performed across different AD stages using a label-free approach.

2018-01-16 | PXD005319 | Pride

An atlas of healthy and injured cell states and niches in the human kidney [Slide-seq2]

Project description:An atlas of healthy and injured cell states and niches in the human kidney [Slide-seq2]

| PRJNA759873 | ENA

Slide-tags: scalable, single-nucleus barcoding for multi-modal spatial genomics

Project description:Recent technological innovations have enabled the high-throughput quantification of gene expression and epigenetic regulation within individual cells, transforming our understanding of how complex tissues are constructed. Missing from these measurements, however, is the ability to routinely and easily spatially localise these profiled cells. We developed a strategy, Slide-tags, in which single nuclei within an intact tissue section are ‘tagged’ with spatial barcode oligonucleotides derived from DNA-barcoded beads with known positions. These tagged nuclei can then be used as input into a wide variety of single-nucleus profiling assays. We used Slide-tags to profile two different stages of development in the mouse brain.

2023-10-03 | GSE244355 | GEO

Analysis of gene expression in glomeruli of BTBR ob/ob diabetic mice

Project description:Analysis of gene expression in glomeruli of BTBR ob/ob diabetic mice

| PRJNA418166 | ENA

Mouse olfactory sensory neurons, main olfactory epithelium and main olfactory bulb

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-01-31 | GSE169021 | GEO