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SUMOylation Regulates Super-Enhancers Through Transcription Factor TFAP2C Binding on Chromatin [RNA-seq]

ABSTRACT: Genome wide localization of SUMO1 and SUMO2/3 proteins revealed an asscociation of SUMO proetins with active chromatin. SUMO proteins are enriched at super enhancers and enhancers and SUMOylation regulates a subset of these super enhancers. Super enhancers regulated by SUMOylation were enriched for transcription factor TFAP2C and SUMOylation negatively regulates TFAP2C localization to enhancers and super enhancers. Proteomics and ChIP-PCR at MYC SE suggests that chromatin bound TFAP2C recruits histone deacetylation complexes that increases upon SAE2 knockdown. Conversely, SUMOylation promoted TFAP2C asscociation with pre-mRNA splicing machinery components. Taken together, our study revealed a critical role of SUMOylation in chromatin modification through an AP-2 family of transcription factor, TFAP2C and a potential role of TFAP2C in pre-mRNA splicing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE171816 | GEO | 2024/04/13

REPOSITORIES: GEO

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SUMOylation Regulates Super-Enhancers Through Transcription Factor TFAP2C Binding on Chromatin [ChIP-seq]

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