Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Developmental chromatin programs determine oncogenic competence in melanoma [Zebrafish]

ABSTRACT: Transcriptional profile of BRAFV600E driven melanoma driven under either the SOX10 or MITFA promoter, both in a p53 deficient background

ORGANISM(S): Danio rerio

PROVIDER: GSE172031 | GEO | 2021/04/15

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Developmental chromatin programs determine oncogenic competence in melanoma [ATAC-seq]

Project description:Oncogenes are only transforming in certain cellular contexts, a phenomenon called oncogenic competence. Here, using a combination of a human pluripotent stem cell-derived cancer model along with zebrafish transgenesis, we demonstrate that the transforming ability of BRAFV600E along with additional mutations depends upon the intrinsic transcriptional program present in the cell of origin. In both systems, melanocytes are largely resistant to mutations, whereas both neural crest and melanoblast populations are readily transformed. Profiling reveals that progenitors have higher expression of chromatin modifying enzymes such as ATAD2, a melanoma competence factor that forms a complex with SOX10 and allows for expression of downstream oncogenic and neural crest programs. These data suggest that oncogenic competence is mediated by regulation of developmental chromatin factors, which then allow for proper response to those oncogenes.

2021-04-15 | GSE172028 | GEO

Developmental chromatin programs determine oncogenic competence in melanoma [Cut&Run]

Project description:We perfomed Cut&Run for ATAD2, MYC and SOX10 in 3xKO ATAD2 dox melanocytes and 3xKO dox melanocytes. We used 10'000 cells per condition and the protocol was performed as in Skene et al., 2018. We could show that, in the 3xKO ATAD2 dox melanocytes, ATAD2, MYC and SOX10 have common targets. Many of those targets belong to a developmental signature typical of the neural crest and to the MAPK pathway.

2021-04-15 | GSE172066 | GEO

Developmental chromatin programs determine oncogenic competence in melanoma.

Project description:Oncogenes only transform cells under certain cellular contexts, a phenomenon called oncogenic competence. Using a combination of a human pluripotent stem cell–derived cancer model along with zebrafish transgenesis, we demonstrate that the transforming ability of BRAFV600E along with additional mutations depends on the intrinsic transcriptional program present in the cell of origin. In both systems, melanocytes are less responsive to mutations, whereas both neural crest and melanoblast populations are readily transformed. Profiling reveals that progenitors have higher expression of chromatin-modifying enzymes such as ATAD2, a melanoma competence factor that forms a complex with SOX10 and allows for expression of downstream oncogenic and neural crest programs. These data suggest that oncogenic competence is mediated by regulation of developmental chromatin factors, which then allow for proper response to those oncogenes.

| S-EPMC9440978 | biostudies-literature

Developmental chromatin programs determine oncogenic competence in melanoma

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-04-15 | GSE172069 | GEO

Developmental chromatin programs determine oncogenic competence in melanoma [RNA-seq]

Project description:Transcriptional profile of human ES-cell (hPSC) derived melanoma cell models

2021-04-15 | GSE172029 | GEO

Developmental chromatin programs determine oncogenic competence in melanoma [RNA-seq II]

Project description:Transcriptional profile of 3xKO dox melanocytes and 3xKO ATAD2 dox melanocytes

2021-04-15 | GSE172030 | GEO

Developmental chromatin programs determine oncogenic competence in melanoma

Project description:Developmental chromatin programs determine oncogenic competence in melanoma

| PRJNA721948 | ENA

Developmental chromatin programs determine oncogenic competence in melanoma [Zebrafish]

Project description:Developmental chromatin programs determine oncogenic competence in melanoma [Zebrafish]

| PRJNA721737 | ENA

MITF deficiency and oncogenic GNAQ each promote proliferation programs in zebrafish melanocyte lineage cells

Project description:Uveal melanoma (UM) is the most common primary malignancy of the eye in adults, but lacks any FDA-approved therapy for the deadly metastatic disease. Thus, there is great need to dissect the driving mechanisms for UM, and develop strategies to evaluate potential therapeutics. Using a zebrafish model, we previously identified MITF, the master melanocyte transcription factor, as a tumor suppressor in GNAQQ209L-driven UM. Here, we show that zebrafish mitfa-deficient GNAQQ209L-driven tumors significantly up-regulate neural crest markers, and higher expression of a melanoma-associated neural crest signature correlates with poor UM patient survival. We further determined how the mitfa-null state, as well as expression of GNAQQ209L, YAPS127A;S381A, or BRAFV600E oncogenes, impacts melanocyte lineage cells before they acquire the transformed state. Specifically, examination 5 days post-fertilization showed that mitfa-deficiency is sufficient to upregulate pigment progenitor and neural crest markers, while GNAQQ209L expression promotes a proliferative phenotype that is further enhanced by YAPS127A;S381A co-expression. Finally, we show that this oncogene-induced proliferative phenotype can be used to screen chemical inhibitors for their efficacy against the UM pathway. Overall, this study establishes that a neural crest signature correlates with poor UM survival, and describes an in vivo assay for pre-clinical trials of potential UM therapeutics.

2022-07-25 | GSE197927 | GEO

Developmental chromatin programs determine oncogenic competence in melanoma [RNA-seq]

Project description:Developmental chromatin programs determine oncogenic competence in melanoma [RNA-seq]

| PRJNA721733 | ENA