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CRISPR screen identifies NUDT21 as a regulator of intron detention of the SAM synthetase MAT2A RNA


ABSTRACT: The only S-adenosymethionine (SAM) synthetase expressed in most human cells, MAT2A, is regulated by intron detention. Using a GFP fusion reporter, we conducted a CRISPR screen to identify regulators of this alternative splicing event. The screen identified METTL16, a known regulator of this process, and NUDT21. NUDT21 encodes the CFIm25 protein a member of the CFIm complex involved in alternative polyadenylation. Validation and follow-up studies support the idea that CFIm25 and the larger CFIm complex plays an unanticipated role in splicing of the MAT2A detained intron.

ORGANISM(S): Homo sapiens

PROVIDER: GSE172217 | GEO | 2021/05/04

REPOSITORIES: GEO

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