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Cardiac and pulmonary responses to diesel engine particulate in Apo E(-/-) mice II

ABSTRACT: Mice deficient in Apolipoprotein E (Apoe E(-/-)) vs wild type mice were exposed to diesel engine particulate by single dose intratracheal instillation. The transcript-level response of the hearts after 24h and of the lungs after 24 h were subsequently analyzed by the 2-color microarray method. Keywords: stress response, disease state analysis

ORGANISM(S): Mus musculus

PROVIDER: GSE17331 | GEO | 2009/10/30

SECONDARY ACCESSION(S): PRJNA119015

REPOSITORIES: GEO

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Cardiac and pulmonary responses to diesel engine particulate in Apo E(-/-) mice II

Project description:Mice deficient in Apolipoprotein E (Apoe E(-/-)) vs wild type mice were exposed to diesel engine particulate by single dose intratracheal instillation. The transcript-level response of the hearts after 24h and of the lungs after 24 h were subsequently analyzed by the 2-color microarray method. Keywords: stress response, disease state analysis Three mice of the Apo E (-/-) strain were exposed to diesel engine particulate from the National Institute of Standards and Technology via intratracheal instillation. Controls consisted of 3 littermates exposed to saline vehicle. Similarly, three wild type mice were similarly exposed to diesel particulate and three to vehicle control. 24 hours after exposure, mice were humanely sacrificed by carbon dioxide asphyxiation and the hearts and lungs were collected and frozen for analysis of total RNA. In each biological replicate, 2 color microarrays were utilized to compare pooled RNA representing all three animals of each strain, and in each condition and in each tissue; each biological replicate was performed with a dye reversal. Biological replicates of the wild type mice responses to diesel engine particulate were performed three times for heart and lung.

2010-06-23 | E-GEOD-17331 | biostudies-arrayexpress

Cardiac and pulmonary responses to diesel engine particulate in Apo E(-/-) mice

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Project description:Diesel engine exhaust (DEE) is one of the major contributors to air pollution around the world, and exposure to DEE is associated with lung cancer and other airway diseases. Although recent studies have investigated the effects of exposure to DEE, the mechanisms by which it leads to lung cancer pathogenesis are not well understood. We have previously investigated the transcriptomic changes that occur due to exposure to cigarette smoke and burning of bituminous (smoky) as well as anthracite (smokeless) coal in the airway epithelium, and in this study we assess the gene expression alterations in the nasal epithelium that are associated with chronic DEE exposure of diesel engine factory workers to better understand which molecular changes may lead to pathogenesis of lung cancer.

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Transcriptomic Changes in the Nasal Epithelium Associated with Diesel Engine Exhaust Exposure

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Proteome-wide changes in primary skin keratinocytes exposed to diesel particulate extract – a role for antioxidants in skin health

Project description:Environmental air pollution such as diesel smoke emission has been shown to have an adverse effect on human skin. Although epidemiological studies showing adverse effects such as oxidative stress-induced aging exists, studies showing the cellular and molecular response to such stress are rare. Using a primary skin keratinocyte model and TMT-based quantitative proteomics strategy, we studied the effects of chronic exposure to diesel particulate matter (DPE) and DPE vapor on the cellular proteome and the ability of a known anti-oxidant Vitamin E in ameliorating such changes. LC-MS3 analysis of DPE and/or its vapor exposed skin keratinocytes resulted in quantification of 4,490 proteins of which 201 and 374 proteins were significantly dysregulated (≥1.5 fold, p-value ≥0.05) in each condition, respectively. We observed distinct molecular alterations in chronic DPE and DPE vapor exposure models. Dysregulation of several cellular processes such as cell cycle and gene regulation, oxidative stress response proteins, skin barrier integrity and skin hydration were observed in both DPE and DPE vapor exposed cells.

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