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Tissue-specific dysregulation of DNA methylation in aging

ABSTRACT: The normal aging process is a complex phenomenon associated with physiological alterations in the function of cells and organs over time. Although an attractive candidate for mediating transcriptional dysregulation, the contribution of epigenetic dysregulation to these progressive changes in cellular function remains unclear. In this study, we employed the genome-wide HELP assay to define patterns of cytosine methylation throughout the rat genome, and the LUMA assay to measure global levels of DNA methylation in the same samples. We studied both liver and visceral adipose tissue, and demonstrated significant differences in DNA methylation with age at >5% of sites analyzed. Furthermore, we showed that epigenetic dysregulation with age is a highly tissue-dependent phenomenon. The most distinctive loci were located at intergenic sequences and conserved non-coding elements, and not at promoters nor at CG-dinucleotide dense loci. Finally, we demonstrated that changes in methylation occur consistently near genes that are involved in metabolism and metabolic regulation, implicating their potential role in the pathogenesis of age-related diseases. We conclude that different patterns of epigenetic dysregulation occur in each tissue over time and may cause some of the physiological changes associated with normal aging.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE17332 | GEO | 2010/05/01

SECONDARY ACCESSION(S): PRJNA119017

REPOSITORIES: GEO

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Tissue-specific dysregulation of DNA methylation in aging

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Rattus norvegicus

Project description:Tissue-specific dysregulation of DNA methylation in aging

| PRJNA119017 | ENA