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Sodium arsenate response of wild type and slt2 deficient cells

ABSTRACT: Wild type (BY4741) Saccharomyces cerevisiae strains and their isogenic slt2 deficient counterparts, were treated for 2 hours with sodium arsenate 100 micromolar. Control (untreated) cells were also collected. Total RNA was extracted and analyzed by microarray hybridization. The data obtained from these experiments allows to determine those genes that are regulated by Slt2 activity after arsenate treatment.

ORGANISM(S): Saccharomyces cerevisiae Schizosaccharomyces pombe

PROVIDER: GSE17364 | GEO | 2010/07/25

SECONDARY ACCESSION(S): PRJNA119087

REPOSITORIES: GEO

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Sodium arsenate response of wild type and slt2 deficient cells

Project description:Wild type (BY4741) Saccharomyces cerevisiae strains and their isogenic slt2 deficient counterparts, were treated for 2 hours with sodium arsenate 100 micromolar. Control (untreated) cells were also collected. Total RNA was extracted and analyzed by microarray hybridization. The data obtained from these experiments allows to determine those genes that are regulated by Slt2 activity after arsenate treatment. The experiments was designed as follows: Four types of samples were used: untreated wild type cells, treated wild type cells, untreated slt2- cells and treated slt2- cells. 3 independent replicates were performed for each experiment.

2010-07-25 | E-GEOD-17364 | biostudies-arrayexpress

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Project description:In our previous work, we had found that Saccharomyces cerevisiae needs of the Hog1 and Slt2 proteins to growth in a low pH environment caused by sulfuric acid, one of the stress factors during the process of ethanol production. Then was performed the gene-wide expression analysis in the hog1M-bM-^HM-^F and slt2M-bM-^HM-^F mutants in order to reveal the function of the Hog1p and Slt2p MAP Kinases in the regulation of S. cerevisiae global gene expression upon stress by sulfuric acid. BY4741 strain (Reference) and their derivate mutants hog1M-bM-^HM-^F and slt2M-bM-^HM-^F were grown for 1 h in YPD medium pH 2.5 adjusted with concentrated sulfuric acid

2013-10-18 | E-GEOD-51353 | biostudies-arrayexpress

Saccharomyces cerevisiae

Project description:Sodium arsenate response of wild type and slt2 deficient cells

| PRJNA119087 | ENA

The role of the Hog1 and Slt2 MAP Kinases in the regulation of Saccharomyces cerevisiae gene expression upon stress by sulfuric acid

Project description:In our previous work, we had found that Saccharomyces cerevisiae needs of the Hog1 and Slt2 proteins to growth in a low pH environment caused by sulfuric acid, one of the stress factors during the process of ethanol production. Then was performed the gene-wide expression analysis in the hog1∆ and slt2∆ mutants in order to reveal the function of the Hog1p and Slt2p MAP Kinases in the regulation of S. cerevisiae global gene expression upon stress by sulfuric acid.

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