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Next-generation sequencing facilitates quantitative analysis of Ym1+/- monocytes from lung transcriptomes

ABSTRACT: Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare Lung Ym1+/- monocytes transcriptome profiling (RNA-seq) to microarray. Methods: Ym1+/- monocytes mRNA profiles of Ym1-Venus mice were generated by deep sequencing, in triplicate, using Illumina NextSeq 500. The sequence reads that passed quality filters were analyzed at the transcript isoform level with methods: TopHat followed by Cufflinks.

ORGANISM(S): Mus musculus

PROVIDER: GSE174199 | GEO | 2021/05/11

REPOSITORIES: GEO

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