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Next generation sequencing facilitates quantitative analysis of colonic tissue from miR-7 deficiency and WT mice with IBD mice, and human colonic epithelial cells transfected-with NC/miR-7 inhibitor/miR-7 inhibitor plus Erlotinib following LPS treatment

ABSTRACT: Methods: Colon tissue from 8-10-Week wild-type (WT) and miR-7 deficiency mice with DSS treatment, and miR-7 inhibitor/NC-transfected-FHC cells and Erlotinib plus miR-7 inhibitor-transfected-FHC cells with LPS treatment, mRNA profiles of which were generated by deep sequencing, using Illumina. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE174331 | GEO | 2023/06/30

REPOSITORIES: GEO

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Next generation sequencing facilitates quantitative analysis of colonic tissue from miR-7 deficiency and WT mice with IBD mice, and human colonic epithelial cells transfected-with NC/miR-7 inhibitor/miR-7 inhibitor plus Erlotinib following LPS treatment

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