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High-resolution mapping of transcribing RNAPII by CRAC in a wt or a sen1T1623E mutant

ABSTRACT: Manuscript title: Modulated termination of non-coding transcription partakes in the regulation of gene expression Here we report high-resolution analyses of transcribing RNAPII in either a wild-type (WT) background or a sen1T1623E mutant, which harbours a substitution that mimics the phosphorylation at threonine 1623. Sen1 is a well-characterized transcription termination factor for RNAPII-dependent non-coding genes in budding yeast. Here we show that the T1623E phosphomimetic mutation induces moderate but widespread termination defects at target non-coding genes, strongly suggesting that phosphoryation at T1623 modulates negatively Sen1 transcription termination activity.

ORGANISM(S): Saccharomyces cerevisiae W303

PROVIDER: GSE174623 | GEO | 2021/10/31

REPOSITORIES: GEO

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