Genomics

Dataset Information

0

The Sen1 Helicase Prevents Spurious Transcription by Controlling Genome-Wide Transcription Termination by RNAPII


ABSTRACT: RNA Polymerase II (RNAPII) termination for transcripts containing a polyadenylation signal (PAS) is thought to differ mechanistically from termination for PAS-independent RNAPII transcripts such as sn(o)RNAs. In a screen for factors required for PAS-dependent termination, we identified Sen1, a putative helicase known primarily for its role in PAS-independent termination. We show that Sen1 is required for termination on hundreds of protein-coding genes and suppresses cryptic transcription from nucleosome-free regions on a genomic scale. These effects often overlap with but are also often distinct from those caused by Nrd1 depletion, which also impacts termination of protein-coding and cryptic transcripts, including many genic antisense transcripts. Sen1 controls termination through its helicase activity and stimulates recruitment of factors previously implicated in both PAS-dependent (Rna14, Rat1) and PAS-independent (Nrd1) termination. Thus, RNAPII termination for both protein-coding genes and cryptic transcripts is dependent on multiple pathways.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE19015 | GEO | 2011/06/01

SECONDARY ACCESSION(S): PRJNA121083

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2011-06-01 | E-GEOD-19015 | biostudies-arrayexpress
2010-01-01 | GSE17559 | GEO
2022-07-13 | GSE174699 | GEO
2015-04-14 | E-GEOD-57155 | biostudies-arrayexpress
2012-11-09 | E-GEOD-39139 | biostudies-arrayexpress
2012-11-09 | GSE39139 | GEO
2015-04-14 | GSE57155 | GEO
2020-01-31 | GSE138465 | GEO
2020-01-31 | GSE138448 | GEO
2020-01-31 | GSE117601 | GEO