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Campylobacter jejuni murine adaptation: unadapted v. adapted

ABSTRACT: Expression arrays comparing Campylobacter jejuni 11168 before and after serial passage in C57 BL/6 IL-10 deficient mice. Gene expression was compared during exponential growth in Bolton broth.

ORGANISM(S): Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 Campylobacter jejuni

PROVIDER: GSE17562 | GEO | 2009/08/08

SECONDARY ACCESSION(S): PRJNA118665

REPOSITORIES: GEO

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Campylobacter jejuni murine adaptation: unadapted v. adapted

Project description:Expression arrays comparing Campylobacter jejuni 11168 before and after serial passage in C57 BL/6 IL-10 deficient mice. Gene expression was compared during exponential growth in Bolton broth. Unpassaged (p0) C. jejuni 11168 was compared to the same strain after 1 passage (p1) and after three passages (p3) through the mice. Biological replicates: 4 for p0 v. p1, 3 for p0 v. p3

2009-08-07 | E-GEOD-17562 | biostudies-arrayexpress

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Campylobacter jejuni murine adaptation: unadapted v. adapted during growth in C57 BL/6 IL-10-/- germ-free mice.

Project description:Expression arrays comparing Campylobacter jejuni 11168 before and after serial passage in C57 BL/6 IL-10 deficient mice. Transcript abundance was compared between C. jejuni variants during growth in the ceca of C57 BL/6 IL-10 deficient germ-free mice. Unpassaged (p0) C. jejuni 11168 was compared to the same strain after three passages (p3) through the mice. Biological replicates: 4; 2 biological replicates were dye swaps.

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Project description:We report the use of differential RNA-sequencing for the determination of the primary transcriptome of wildtype Campylobacter jejuni NCTC 11168. This allows for the genome-wide determination of transcription start sites.

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Differential RNA-sequencing of Campylobacter jejuni fur perR mutant

Project description:We report the use of differential RNA-sequencing for the determination of the primary transcriptome of the fur perR mutant of Campylobacter jejuni NCTC 11168. This allows for the genome-wide determination of transcription start sites.

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Gene Expression Changes in Campylobacter jejuni triggered by exposure to Novobiocin

Project description:In order to investigate the gene experssion changes in Campylobacter jejuni triggered by exposure to Novobiocin. DNA microarray was used to identify genes that were differentially expressed in C. jejuni 11168 treated with Novobiocin. For RNA extraction, Campylobacter cells were grown 4 hrs in MH broth to the mid exponential phase (OD600=0.4-0.5) with shaking rates 180rpm at 42Â°C, and then split into two equal portions, one of which was treated with subinhibitory dose of Novobiocin (256ug/ml, 0.25 MIC) and the other served as a non-treated control, all of these samples were incubated at 42Â°C for 30 min under microaerobic conditions, RNA samples were extracted from 4 independent treatments and 4 non-treated controls. Then cDNA was synthesized using aminoallyl-dNTP and RNA samples according to the manufacturerâs instruction. After purification, aminoallyl labeled cDNA was fluorescently labeled with Cy-3/Cy-5 mono-Reactive Dye Pack.

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