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Adipogenic differentiation of gluteal and abdominal adipose stem cells

ABSTRACT: Obesity and associated metabolic outcomes define the metabolic syndrome. Interestingly, an under-appreciated fact is that body fat distribution, rather than total body fat amount, is a key determinant of metabolic disease. Indeed, in contrast to upper-body obesity, lower-body fat accumulation inversely correlates with metabolic risks. Understanding processes regulating upper- vs. lower-body fat expansion is paramount to predict (and prevent) these risks. We combine functional, proteomics, transcriptomics and epigenomics analyses to identify chromatin-associated mechanisms of adipose depot-specific fat expansion. Here, we analyze by RNA-seq the transcriptome of adipose stem cells (ASCs) from gluteal (lower-body) and abdominal subcutaneous (upper-body) depots induced to differentiate in vitro towards the adipogenic lineage. We aim to identify adipose depot-specific and temporal differences in the up- or down-regulation of gene expression, and a later stage relate these differences to changes in chromatin states.

ORGANISM(S): Homo sapiens

PROVIDER: GSE176020 | GEO | 2022/06/17

REPOSITORIES: GEO

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