Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-Seq analysis of Sfmbt depletion and ChIP-Seq analysis of Pho and Sfmbt-GFP binding in Drosophila ovaries

ABSTRACT: We analyzed the transcriptome of young ovaries by RNA sequencing (RNAseq) after germ line depletion of Sfmbt by RNAi. We determined the genomic binding sites for GFP-Sfmbt and Pho using ChIPseq.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE176034 | GEO | 2022/09/22

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA-Seq analysis of Sfmbt depletion and ChIP-Seq analysis of Pho and Sfmbt-GFP binding in Drosophila ovaries

Project description:RNA-Seq analysis of Sfmbt depletion and ChIP-Seq analysis of Pho and Sfmbt-GFP binding in Drosophila ovaries

| PRJNA734622 | ENA

Stenotrophomonas sp. pho isolate:pho

Project description:Stenotrophomonas sp. pho isolate:pho Genome sequencing

| PRJNA473395 | ENA

Protein interactions within PLEIOHOMEOTIC-repressive complex are critical for its recruitment to Polycomb target genes (Fruit Fly)

Project description:PcG proteins are critical epigenetic regulators of development. Here we have determined genomic distributions of PHO, PHOL and SFMBT proteins in cultured Drosophila cells by hybridization of ChIP products with tiling microarrays. genomic distributions of PHO, PHOL and SFMBT proteins in cultured Drosophila cells by hybridization of ChIP products with tiling microarrays.

2014-06-07 | E-GEOD-41852 | biostudies-arrayexpress

Blastocladiella PHO system

Project description:Blastocladiella PHO system

| PRJEB20783 | ENA

Protein interactions within PLEIOHOMEOTIC-repressive complex are critical for its recruitment to Polycomb target genes (Fruit Fly)

2014-06-07 | GSE41852 | GEO

Transcriptional profiles of sov mutant ovaries

Project description:Stranded, single-end, polyA+, transcriptional profiles were created from ovaries of sterile and fertile sov heteroallelic mutants and Gal4 driven sov RNAi knockdowns.

2019-01-01 | GSE113977 | GEO

Context dependent role of Pho binding sites in Polycomb complex recruitment in Drosophila

Project description:Polycomb group (PcG) proteins maintain the silenced state of key developmental genes, but how these proteins are recruited to specific regions of the genome is still not completely understood. In Drosophila, PcG proteins are recruited to Polycomb response elements (PREs) comprised of a flexible array of sites for sequence-specific DNA binding proteins, “PcG recruiters”, including Pho, Spps, Cg, GAF and many others. Pho is thought to play a central role in PcG recruitment. Early data showed that mutation of Pho binding sites in PREs in transgenes abrogated the ability of those PREs to repress gene expression. In contrast, genome-wide experiments in pho mutants or by Pho knockdown showed that PcG proteins can bind to PREs in the absence of Pho. Here we directly addressed the importance of Pho binding sites in two engrailed (en) PREs at the endogenous locus and in transgenes. Our results show that Pho binding sites are required for PRE activity in transgenes with a single PRE. In a transgene, two PREs together lead to stronger, more stable repression and confer some resistance to the loss of Pho binding sites. Making the same mutation in Pho binding sites has little effect on PcG-protein binding at the endogenous en gene. Overall, our data support the model that Pho is important for PcG binding but emphasize how multiple PREs and chromatin environment increase the ability of PREs to function in the absence of Pho.

2023-05-08 | GSE227553 | GEO

Genome-wide gene expression profiling of rice Indica cultivar Zhongxian 3037 and mutant phoenix (pho) panicle

Project description:phoenix (pho) is a naturally occurring rice (Oryza sativa L.) mutant that exhibits stable inflorescence reversion. Genetic analysis showed that it was not a simple mendelian mutant. In order to find the major genes contributed to the formation of pho, microarray experiments were carried out to analyze and compare the transcriptional profiles between pho and wild-type panicles.

2009-07-22 | GSE17194 | GEO

Genome-wide gene expression profiling of rice Indica cultivar Zhongxian 3037 and mutant phoenix (pho) panicle

2009-07-22 | E-GEOD-17194 | biostudies-arrayexpress

Global gene expression comparation among testes, ovaries, Smad4flox/floxMerCreMer Stra8+/− ovaries and Smad4flox/floxMerCreMer Stra8−/− ovaries

Project description:To confirm that female-to-male sexual fate reversal in Smad4flox/floxMerCreMer Stra8−/− ovaries occurs independently of somatic environment. We analyzed transcriptome of samples using RNA from control testes, ovaries, Smad4flox/floxMerCreMer Stra8+/− and Smad4flox/floxMerCreMer Stra8−/− ovaries.

2016-05-11 | GSE68773 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data