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RNA-Seq analysis of Sfmbt depletion and ChIP-Seq analysis of Pho and Sfmbt-GFP binding in Drosophila ovaries

ABSTRACT: RNA-Seq analysis of Sfmbt depletion and ChIP-Seq analysis of Pho and Sfmbt-GFP binding in Drosophila ovaries

PROVIDER: PRJNA734622 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14713176_1.fastq.gz	Fastqsanger.gz
	SRR14713176_2.fastq.gz	Fastqsanger.gz
	SRR14713177_1.fastq.gz	Fastqsanger.gz
	SRR14713177_2.fastq.gz	Fastqsanger.gz
	SRR14713178_1.fastq.gz	Fastqsanger.gz

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RNA-Seq analysis of Sfmbt depletion and ChIP-Seq analysis of Pho and Sfmbt-GFP binding in Drosophila ovaries

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Project description:Polycomb group (PcG) proteins maintain the silenced state of key developmental genes, but how these proteins are recruited to specific regions of the genome is still not completely understood. In Drosophila, PcG proteins are recruited to Polycomb response elements (PREs) comprised of a flexible array of sites for sequence-specific DNA binding proteins, “PcG recruiters”, including Pho, Spps, Cg, GAF and many others. Pho is thought to play a central role in PcG recruitment. Early data showed that mutation of Pho binding sites in PREs in transgenes abrogated the ability of those PREs to repress gene expression. In contrast, genome-wide experiments in pho mutants or by Pho knockdown showed that PcG proteins can bind to PREs in the absence of Pho. Here we directly addressed the importance of Pho binding sites in two engrailed (en) PREs at the endogenous locus and in transgenes. Our results show that Pho binding sites are required for PRE activity in transgenes with a single PRE. In a transgene, two PREs together lead to stronger, more stable repression and confer some resistance to the loss of Pho binding sites. Making the same mutation in Pho binding sites has little effect on PcG-protein binding at the endogenous en gene. Overall, our data support the model that Pho is important for PcG binding but emphasize how multiple PREs and chromatin environment increase the ability of PREs to function in the absence of Pho.

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