Large scale analysis of leaf gene expression upon treatment of apple seedlings with acibenzolar-S-methyl or water
Ontology highlight
ABSTRACT: Investigation of whole genome gene expression level changes in the youngest expanded leaves of open-pollinated Golden Delicious seedlings either sprayed with reverse osmosis water or acibenzolar-S-methyl (ASM). Treatment was performed by spraying sprayed to runoff (with a pressurized hand sprayer) with the commercial product Bion 50 WG (Syngenta, Basel, Switzerland; 50% of ASM) prepared in reverse osmosis water at a final concentration of 0.4 g/L. The youngest developped leaf of each seedling was sampled 3 days after the treatment.
Project description:Investigation of whole genome gene expression level changes in the youngest expanded leaves of GDDH13 grafted scions either sprayed with reverse osmosis water or acibenzolar-S-methyl (ASM) either exposed to control temperature (21°C/day ; 18°C/night) or three successive heat days (35°C for 4 h during the day ; 18°C/night). The morning of the third heat day, treatment was performed by spraying to runoff (with a pressurized hand sprayer) with the commercial product Bion 50 WG (Syngenta, Basel, Switzerland; 50% of ASM) prepared in reverse osmosis water at a final concentration of 0.4 g/L. All plants were then returned to control thermal conditions (21°C/day ; 18°C/night) for 2 days. The youngest developped leaf of each plant was sampled 72 hours after the treatment.
Project description:Investigation of whole genome gene expression level changes in leaves of apple seedlings (Golden delicious) 3 days after treatment by Bion 50WG (ASM) versus water.
Project description:The transcriptome of P. aeruginosa PA01 biofilm cells was compared to the associated suspended culture, upon growth on reverse osmosis membrane coupon under limited nutrient conditions. Keywords: growth in reverse osmosis unit and on reverse osmosis membrane
Project description:The goal of this project was to investigate GSTs labelled by GST-G probe in non-treated Arabidopsis (Water samples: Click control vs GST-G) or GSTs activated upon Bion treatment of Arabidopsis (Water GST-G vs Bion GST-G).