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Effect of Bion (BTH treatment) in GST activities on 6-week old A.thaliana plants

ABSTRACT: The goal of this project was to investigate GSTs labelled by GST-G probe in non-treated Arabidopsis (Water samples: Click control vs GST-G) or GSTs activated upon Bion treatment of Arabidopsis (Water GST-G vs Bion GST-G).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Farnusch Kaschani

LAB HEAD: Farnusch Kaschani

PROVIDER: PXD045884 | Pride | 2024-01-26

REPOSITORIES: Pride

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			Action	DRS
	ACE_0565_MF01.raw	Raw
	ACE_0565_MF02.raw	Raw
	ACE_0565_MF03.raw	Raw
	ACE_0565_MF04.raw	Raw
	ACE_0565_MF05.raw	Raw

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Publications

Glutathione Transferase Photoaffinity Labeling Displays GST Induction by Safeners and Pathogen Infection.

Font Farre Maria M Brown Daniel D König Maurice M Killinger Brian J BJ Kaschani Farnusch F Kaiser Markus M Wright Aaron T AT Burton Jonathan J van der Hoorn Renier A L RAL

Plant & cell physiology 20240101 1

Glutathione transferases (GSTs) represent a large and diverse enzyme family involved in the detoxification of small molecules by glutathione conjugation in crops, weeds and model plants. In this study, we introduce an easy and quick assay for photoaffinity labeling of GSTs to study GSTs globally in various plant species. The small-molecule probe contains glutathione, a photoreactive group and a minitag for coupling to reporter tags via click chemistry. Under UV irradiation, this probe quickly an ...[more]

PMID: 37924215

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Transcription profiling of Arabidopsis mutants (elo2, elo3, drl1-2 and ang4) vs the wild type Ler strain

Project description:Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization between complementary nucleic acid strands, inciting us to create a collection of gene-specific sequence tags (GSTs) representing at least 21,500 Arabidopsis genes and which are compatible with both approaches. The GSTs were carefully selected to ensure that each of them shared no significant similarity with any other region in the Arabidopsis genome. They were synthesized by PCR amplification from genomic DNA. Spotted microarrays fabricated from the GSTs show good dynamic range, specificity and sensitivity in transcript profiling experiments. The GSTs have also been transferred to bacterial plasmid vectors via recombinational cloning protocols. These cloned GSTs constitute the ideal starting point for a variety of functional approaches, including reverse genetics. We have subcloned GSTs on a large scale into vectors designed for gene silencing in plant cells. We show that in planta expression of GST hairpin RNA results in the expected phenotypes in silenced Arabidopsis lines. These versatile GST resources provide novel and powerful tools for functional genomics. Supplemental material is available online at www.genome.org. The sequence data have been submitted to GenBank under accession number XXXXX. The GST sequence information is accessible via the CATMA database (www.catma.org) and other Arabidopsis databases, the corresponding CATMA array, or corresponding CATMA-based transcript profiling service, are available from multiple genomics facilities and the GST clone repertoire will be distributed by the Nottingham Arabidopsis Stock Centre (nasc.nott.ac.uk).

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Confirmation of replication interactome of TopBP1 using proximity-dependent biotinylation MS

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Ubiquitin-independent disassembly by a p97 AAA-ATPase complex drives PP1 holoenzyme formation

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