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Fully characterized mature human iPS- and NMP-derived motor neurons thrive without neuroprotection in the spinal contusion cavity [SMN V3]

ABSTRACT: Unpublished findings related to Frontiers 2021 article: Fully characterized mature human iPS- and NMP-derived motor neurons thrive without neuroprotection in the spinal contusion cavity

ORGANISM(S): Homo sapiens

PROVIDER: GSE178600 | GEO | 2021/10/14

REPOSITORIES: GEO

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Fully characterized mature human iPS- and NMP-derived motor neurons thrive without neuroprotection in the spinal contusion cavity [SMN V3]

Project description:Fully characterized mature human iPS- and NMP-derived motor neurons thrive without neuroprotection in the spinal contusion cavity [SMN V3]

| PRJNA739709 | ENA

Fully Characterized Mature Human iPS- and NMP-Derived Motor Neurons Thrive Without Neuroprotection in the Spinal Contusion Cavity.

Project description:Neural cell interventions in spinal cord injury (SCI) have focused predominantly on transplanted multipotent neural stem/progenitor cells (NSPCs) for animal research and clinical use due to limited information on survival of spinal neurons. However, transplanted NSPC fate is unpredictable and largely governed by injury-derived matrix and cytokine factors that are often gliogenic and inflammatory. Here, using a rat cervical hemicontusion model, we evaluate the survival and integration of hiPSC-derived spinal motor neurons (SMNs) and oligodendrocyte progenitor cells (OPCs). SMNs and OPCs were differentiated in vitro through a neuromesodermal progenitor stage to mimic the natural origin of the spinal cord. We demonstrate robust survival and engraftment without additional injury site modifiers or neuroprotective biomaterials. Ex vivo differentiated neurons achieve cervical spinal cord matched transcriptomic and proteomic profiles, meeting functional electrophysiology parameters prior to transplantation. These data establish an approach for ex vivo developmentally accurate neuronal fate specification and subsequent transplantation for a more streamlined and predictable outcome in neural cell-based therapies of SCI.

| S-EPMC8762343 | biostudies-literature

Mature human iPS- and NMP-derived motor neurons in alginate neural ribbons [Alg60]

Project description:Unpublished findings related to iScience 2021 article: Fully characterized mature human iPS- and NMP-derived motor neurons thrive without neuroprotection in the spinal contusion cavity

2022-05-06 | GSE178601 | GEO

Transcription profiling of rat contusion model treated with clenbuterol, X-ray and tempol

Project description:Gene expression analysis after the treatments for functional recovery after contusion injury which are mediated by glutathione Experiment Overall Design: 24 hours after contusion and treatments Experiment Overall Design: Groups include normal, laminectomy, contusion and treatments on contused rats (clenbuterol, X-ray, tempol, BSO and tempol/BSO)

2008-06-13 | E-GEOD-5273 | biostudies-arrayexpress

Normothermic machine perfusion (NMP) inhibits proinflammatory responses in the liver and promotes regeneration

Project description:Background and Aims: Liver transplantation is a successful treatment for patients with liver failure. However, organ shortage results in over 11% of patients loosing their chance of a transplant due to worsening of their liver disease. Ischemia/reperfusion injury (IRI) is the major cause leading to graft loss after liver transplantation. Therefore, novel methods of organ-preservation have been developed in recent years to minimize IRI. One such device is the OrganOx metra, a normothermic machine perfusion (NMP) device providing oxygen and nutrition to allow aerobic metabolism and minimizing IRI. OrganOx metra has presented with several advantages compared to conventional cold storage (CS). We aimed to confirm the impact of NMP on reducing IRI and to define the underling mechanisms. Methods: We compared 12 NMP with 27 CS-preserved livers by performing gene microarray, immunoprofiling of hepatic lymphocytes and immunochemistry staining of liver tissues for assessing necrosis, platelet deposition and neutrophil infiltration, and the status of steatosis after NMP or CS pre- and post-reperfusion. Results: Recipients receiving NMP grafts showed significantly lower peak AST levels than those receiving CS grafts. NMP altered gene-expression profiles of liver tissue from pro-inflammation to pro-healing and regeneration. NMP also reduced the number of IFN- and IL-17 producing T cells and enlarged CD4posCD25highCD127negFOXP3pos Treg pool. NMP liver tissues showed less necrosis and apoptosis in the parenchyma and fewer neutrophils and platelet infiltration compared to CS liver tissues. Conclusion: Reduced IRI in NMP recipients was the consequence of the combination of inhibiting inflammation and promoting graft regeneration.

2019-04-10 | GSE112713 | GEO

Chronic contusion spinal cord injury in rats

Project description:Excerpt from a larger study which characterized the transcriptional effects of a spinal cord contusion injury in rats. This is the data from the almost chronic contusion state (35 days) at the injury site (Thoracic 8) - where we saw significant changes in several areas, including cholesterol metabolism genes. Other spinal cord areas (rostral, caudal) and time-points (3 hours, 24 hours, 7 days and 35 days) were analyzed as well and are discussed in our paper and at www.crpf.org/microarray. Keywords = Spinal Cord Injury Keywords = chronic Keywords = thoracic Keywords = cholesterol Keywords: repeat sample

2005-05-03 | GSE2599 | GEO

Transcription profiling of rat spinal cord contusion 35 days after injury

2007-10-08 | E-GEOD-2599 | biostudies-arrayexpress

RAT liver OXYGEN-VENTILATED NMP LC-MS/MS

Project description:The project was to compare NMP on liver retrieval in DCD rats.

2022-05-19 | PXD028641 | Pride

Clenbuterol, X-ray and Tempol treatments on Contusion Rat model

Project description:Gene expression analysis after the treatments for functional recovery after contusion injury which are mediated by glutathione Keywords: treatment

2006-07-12 | GSE5273 | GEO

Single cell RNA sequencing of in vitro differentiation NMP protocols

Project description:The mammalian embryos Caudal Lateral Epiblast (CLE) harbours bipotent progenitors, called Neural Mesodermal Progenitors (NMPs), that contribute to the spinal cord and the paraxial mesoderm throughout axial elongation. Here we performed a single cell analysis of different in vitro NMPs populations produced either from embryonic stem cells (ESCs) or epiblast stem cells (EpiSCs) and compared them to E8.25 CLE mouse embryos. In our analysis of this region our findings challenge the notion that NMPs can be defined by the exclusive coexpression of Sox2 and T at mRNA level. We analyse the in vitro NMP-like populations using a purpose-built Support Vector Machine (SVM) based on the embryo CLE and use it as a classification model to compare the in vivo and in vitro populations. Our results show that NMP differentiation from ESCs, leads to heterogeneous progenitor populations with few NMP-like cells, as defined by the SVM algorithm, whereas starting with EpiSCs, yields a high proportion of cells with the embryo NMP signature. We find that the population from which the Epi-NMPs are derived in culture contains a node-like population, which leads to suggest that this population probably maintains the expression of T in vitro and thereby a source of NMPs. In conclusion, differentiation of EpiSCs into NMPs reproduces events in vivo and suggests a sequence of events for the emergence of the NMPs population.

2019-06-11 | GSE132504 | GEO

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