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Fully characterized mature human iPS- and NMP-derived motor neurons thrive without neuroprotection in the spinal contusion cavity [SMN V3]

ABSTRACT: Fully characterized mature human iPS- and NMP-derived motor neurons thrive without neuroprotection in the spinal contusion cavity [SMN V3]

PROVIDER: PRJNA739709 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14872435_1.fastq.gz	Fastqsanger.gz
	SRR14872435_2.fastq.gz	Fastqsanger.gz
	SRR14872436_1.fastq.gz	Fastqsanger.gz
	SRR14872436_2.fastq.gz	Fastqsanger.gz
	SRR14872437_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Fully characterized mature human iPS- and NMP-derived motor neurons thrive without neuroprotection in the spinal contusion cavity [SMN V3]

Project description:Unpublished findings related to Frontiers 2021 article: Fully characterized mature human iPS- and NMP-derived motor neurons thrive without neuroprotection in the spinal contusion cavity

2021-10-14 | GSE178600 | GEO

Mature human iPS- and NMP-derived motor neurons in alginate neural ribbons [Alg60]

Project description:Unpublished findings related to iScience 2021 article: Fully characterized mature human iPS- and NMP-derived motor neurons thrive without neuroprotection in the spinal contusion cavity

2022-05-06 | GSE178601 | GEO

Fully Characterized Mature Human iPS- and NMP-Derived Motor Neurons Thrive Without Neuroprotection in the Spinal Contusion Cavity.

Project description:Neural cell interventions in spinal cord injury (SCI) have focused predominantly on transplanted multipotent neural stem/progenitor cells (NSPCs) for animal research and clinical use due to limited information on survival of spinal neurons. However, transplanted NSPC fate is unpredictable and largely governed by injury-derived matrix and cytokine factors that are often gliogenic and inflammatory. Here, using a rat cervical hemicontusion model, we evaluate the survival and integration of hiPSC-derived spinal motor neurons (SMNs) and oligodendrocyte progenitor cells (OPCs). SMNs and OPCs were differentiated in vitro through a neuromesodermal progenitor stage to mimic the natural origin of the spinal cord. We demonstrate robust survival and engraftment without additional injury site modifiers or neuroprotective biomaterials. Ex vivo differentiated neurons achieve cervical spinal cord matched transcriptomic and proteomic profiles, meeting functional electrophysiology parameters prior to transplantation. These data establish an approach for ex vivo developmentally accurate neuronal fate specification and subsequent transplantation for a more streamlined and predictable outcome in neural cell-based therapies of SCI.

| S-EPMC8762343 | biostudies-literature

Transcription profiling of rat contusion model treated with clenbuterol, X-ray and tempol

Project description:Gene expression analysis after the treatments for functional recovery after contusion injury which are mediated by glutathione Experiment Overall Design: 24 hours after contusion and treatments Experiment Overall Design: Groups include normal, laminectomy, contusion and treatments on contused rats (clenbuterol, X-ray, tempol, BSO and tempol/BSO)

2008-06-13 | E-GEOD-5273 | biostudies-arrayexpress

Trancriptional regulation by axonal SMN on motor neuron cells

Project description:Muscular atrophy (SMA) is an autosomal recessive disease causing selective motor neuron death by the loss of telomeric survival motor neuron gene, SMN1. Axonal SMN, a-SMN, is a truncated form of SMN, derived from an alternatively spliced SMN1 gene. (Setola, et. al. 2007 PNAS 104, 1959-1964). The cellular clones expressing a-SMN in a tetracycline-dependent manner were isolated from NSC34 by two-step stable transfection, first with the tetracycline-repressor construct and subsequently with the a-SMN cDNA. To identify novel a-SMN target genes, the transcriptome of several a-SMN clones was analyzed and compared with that of parental cells.

2012-01-07 | E-MEXP-3402 | biostudies-arrayexpress

Normothermic machine perfusion (NMP) inhibits proinflammatory responses in the liver and promotes regeneration

Project description:Background and Aims: Liver transplantation is a successful treatment for patients with liver failure. However, organ shortage results in over 11% of patients loosing their chance of a transplant due to worsening of their liver disease. Ischemia/reperfusion injury (IRI) is the major cause leading to graft loss after liver transplantation. Therefore, novel methods of organ-preservation have been developed in recent years to minimize IRI. One such device is the OrganOx metra, a normothermic machine perfusion (NMP) device providing oxygen and nutrition to allow aerobic metabolism and minimizing IRI. OrganOx metra has presented with several advantages compared to conventional cold storage (CS). We aimed to confirm the impact of NMP on reducing IRI and to define the underling mechanisms. Methods: We compared 12 NMP with 27 CS-preserved livers by performing gene microarray, immunoprofiling of hepatic lymphocytes and immunochemistry staining of liver tissues for assessing necrosis, platelet deposition and neutrophil infiltration, and the status of steatosis after NMP or CS pre- and post-reperfusion. Results: Recipients receiving NMP grafts showed significantly lower peak AST levels than those receiving CS grafts. NMP altered gene-expression profiles of liver tissue from pro-inflammation to pro-healing and regeneration. NMP also reduced the number of IFN- and IL-17 producing T cells and enlarged CD4posCD25highCD127negFOXP3pos Treg pool. NMP liver tissues showed less necrosis and apoptosis in the parenchyma and fewer neutrophils and platelet infiltration compared to CS liver tissues. Conclusion: Reduced IRI in NMP recipients was the consequence of the combination of inhibiting inflammation and promoting graft regeneration.

2019-04-10 | GSE112713 | GEO

HITS-CLIP of SMN

Project description:HITS-CLIP of SMN

| PRJNA433653 | ENA

Transcriptome profiling of untreated and doxycycline-treated HeLa/TO-SmN

Project description:Purpose is to examine the effect of SmN expression to gene expression in HeLa Compare the differences between the transcriptome of untreated and doxycycline-treated HeLa/TO-SmN in which expression of SmN is under doxycycline control

2015-01-16 | E-GEOD-54136 | biostudies-arrayexpress

Chronic contusion spinal cord injury in rats

Project description:Excerpt from a larger study which characterized the transcriptional effects of a spinal cord contusion injury in rats. This is the data from the almost chronic contusion state (35 days) at the injury site (Thoracic 8) - where we saw significant changes in several areas, including cholesterol metabolism genes. Other spinal cord areas (rostral, caudal) and time-points (3 hours, 24 hours, 7 days and 35 days) were analyzed as well and are discussed in our paper and at www.crpf.org/microarray. Keywords = Spinal Cord Injury Keywords = chronic Keywords = thoracic Keywords = cholesterol Keywords: repeat sample

2005-05-03 | GSE2599 | GEO

HITS-CLIP of SMN

Project description:We performed HITS-CLIP to determine cellular RNA targets of the SMN protein. Three replicates were performed in SH-SY5Y cells.

2018-08-01 | GSE110411 | GEO

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