Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Stem Cell transcriptional response to Microglia-Conditioned Media

ABSTRACT: This paper demonstrates that whereas SOX2+ cells in HDB are highly susceptible to ZKV, SOX2+ primary GBM samples exhibit moderate to high resistance to infection. Expression profiling revealed an innate immune signature due to microglia infiltration in highly resistant GBM samples that was lacking in HDB. This data was generated to proflile the transcriptional response to microglia-secreted factors in stem cells from glioma cell lines and human developing brain. We find that Interferon-stimulated genes are the main targets upregulated in response to microglia conditioned media exposure.

ORGANISM(S): Homo sapiens

PROVIDER: GSE178620 | GEO | 2022/09/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Microglia regulate Zika virus infection in human developing brain and glioma

Project description:This paper shows that, whereas SOX2+ cells in HDB are highly susceptible to ZKV, SOX2+ primary GBM samples exhibit moderate to high resistance to infection. These datasets address the reasons for this difference, based on infection of primary patient GBM and human developing brain samples with mCherry Zika reporter virus, then FACS sorting the Zika-mCherry positive and negative fractions from each sample for bulk RNA-Sequencing. The primary finding of importance is that Zika resistant GBMs exhibit an immune signature reflecting high levels of infiltration by microglia which are present only in small numbers or absent altogether in human developing brain.

2022-09-08 | GSE178621 | GEO

RNA-Sequencing of Astrocytes Stimulated with Activated Endothelial conditioned media (ECM) and Activated Microglia conditioned media (MCM)

Project description:The goal of this study is to compare the mRNA transcriptome of astrocytes treated with Activated Microglia and Endothelial conditioned media

2022-03-18 | GSE175485 | GEO

RNA-Sequencing of Astrocytes Stimulated with Activated Endothelial conditioned media (ECM) and Activated Microglia conditioned media (MCM)

Project description:RNA-Sequencing of Astrocytes Stimulated with Activated Endothelial conditioned media (ECM) and Activated Microglia conditioned media (MCM)

| PRJNA732462 | ENA

NRVM Conditioned Media Proteomics

Project description:Proteomic results from NRVM conditioned media overexpressing GRK2

2024-04-22 | PXD050356 | Pride

Immune surveillance of brain metastatic cancer cells is mediated by IFITM1 (Proteins identified in the >100 kDa fraction of conditioned media from all four cell lines)

Project description:The protein(s) larger than 100 kDa in conditioned medium from lung cancer cell lines activated microglia. The >100 kDa fraction of conditioned medium from H460, H1299, H2030, or H292 cells was collected. Proteins present in the conditioned medium were analyzed by LC-MS/MS.

2023-02-17 | PXD038129 | Pride

Microglia treatment with C6 glioma-conditioned medium or with LPS

Project description:Several independent microglia preparations performed on different days were plated onto wells of 24-well plates, and either left untreated (control), treated with the C6 glioma-conditioned medium, or treated with LPS.<br>For each culture condition, a number of RNA samples were prepared, with each sample then separately labeled and hybridized. These RNA samples were prepared from non-overlapping sets of wells from at least two microglia preparations, so they constitute biological replicates.

2012-12-14 | E-MEXP-2466 | biostudies-arrayexpress

Time-series transcription profiling by array of rat microglia treated with C6 glioma-conditioned medium.

Project description:Several independent microglia preparations performed on different days were plated onto wells of 24-well plates, and either left untreated (control), treated with the C6 glioma-conditioned medium (GCM). RNA samples were prepared at 6, 24 and 48 hours from the start of the treatment, as well as from the control untreated microglia, with each sample then separately labeled and hybridized. These RNA samples were prepared from from at three independent microglia preparations, so they constitute biological replicates.

2018-10-03 | E-MTAB-6143 | biostudies-arrayexpress

Metabolomics of Adipocyte-Conditioned Media Compared to Stromal Cell- and Un-conditioned Media

Project description:The metabolomics profiles of adipocyte conditioned media (ACM), stromal cell conditioned media (SCM) and unconditioned media (UCM) were analyzed by untargeted mass spectrometry.

2022-10-21 | ST002399 | MetabolomicsWorkbench

Expression data from murine mice microglia treated with Glioma Conditioned Medium for 6h

Project description:Immune cells accumulating in the microenvironment of malignant tumors are tumor-educated, and contribute to its growth, progression and evasion of antitumor immune responses. Glioblastoma (GBM), the common and most malignant primary brain tumor in adults, shows considerable accumulation of resident microglia and peripheral macrophages, and their polarization into tumor-supporting cells. There are controversies regarding a functional phenotype of glioma-associated microglia/macrophages (GAMs) due to a lack of consistent markers. Previous categorization of GAM polarization towards the M2 phenotype has been found inaccurate because of oversimplification of highly complex and heterogeneous responses. In this study we characterized functional responses and gene expression in mouse and human microglial cultures exposed to fresh conditioned media (GCM) from human U87 and LN18 glioma cells. Functional analyses revealed mutual communication reflected by strong stimulation of glioma invasion by microglial cells and increased microglial phagocytosis after GCM treatment. To define transcriptomic markers of GCM-activated microglia, we performed selected and global gene expression analyses of stimulated microglial cells. We found activated pathways associated with immune-evasion and TGF signaling. We performed computational comparison of the expression patterns of GAMs from human GBMs and rodent experimental gliomas to select genes consistently changed in different datasets. The analyses of marker genes in GAMs from different experimental models and clinical samples revealed only a small set of common genes, which reflects variegated responses in clinical and experimental settings. We discuss potential sources of the observed differences and stress a great need for definitive elucidation of a functional state of GAMs.

2018-04-20 | GSE113370 | GEO

Human osteoblast treated with chemotherapy-treated bone marrow stromal cell conditioned media

Project description:Global expression profile of human osteoblast treated with chemotherapy-treated bone marrow stromal cell conditioned media, compared to human osteoblast cells treated with diluent-control bone marrow stromal cell conditioned media. Goal is to identify genes regulated by chemotherapy in osteoblasts. Dye-swap design with 6 biological replicates. Three arrays performed with chemotherapy treated samples on channel 1 and diluent-control treated on channel 2; three arrays performed with chemotherapy treated samples on channel 2 and diluent-control on channel 1.

2010-09-30 | E-GEOD-17842 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data