Project description:We report transcriptomes of cells from large skin wounds. Dead cells were removed from post-wounding day (PWD) 12 skin wound tissue using dead cell removal kit (MACS). Remaining live cells were analyzed
Project description:Standardized skin wounds were established surgically on mice and allowed to heal during a 15-day period. Expression of genes related to heparan sulfate biosynthesis was studied in wound bed and edges during the healing process. Total RNA was isolated from wound edge (regenerating skin) and wound bed at 2, 6 and 15 days post wounding, as well as from intact control skin. Three animals were used for each time point.
Project description:Wound healing is orchestrated by a spatial and temporal network of intercellular communication between epithelial cells, the stromal compartment, and the immune system. Our scRNA-seq analysis of Hgfac WT and KO wounds reveals that Aldh1a3 is downstream of the HGFAC system and Aldh1a3 is highly upregulated in wound-asociated epithelial (WAE) cells in responce to endoscopic wounding, suggesting WAE cells can potentially secrete retinoic acid into wound bed. To dissect the healing responce in the intestinal mucosa, we peformed spatial transcriptomics analysis.
Project description:To investigate genes that are involved in hematopoietic cells production in vivo, we used microarray technology combined with a transgenic mouse model IIb-tk previously developed in our laboratory (Tropel et al., 1997). In these mice, the promoter region of the murine CD41 (GPIIb) gene was used to target the expression of the thymidine kinase (tk) toxigene in cells specifically expressing the CD41 gene. The strength of the model resides in the fact that the depletion of the entire myelo-megakaryocytic lineage including stem cells/early progenitors by ganciclovir (GCV) administration could be inverted by discontinuing the treatment. A nearly full recovery of mature myelo-megakaryocytic cells is then observed within 3 days. To determine at the molecular level how BM cells could be regenerated in vivo following the eradication of all myeloid progenitors by the GCV treatment, microarray technology was applied. The strategy involved monitoring early transcriptional changes for the first 3-day of BM regeneration. Keywords: time-course
Project description:To investigate genes that are involved in hematopoietic cells production in vivo, we used microarray technology combined with a transgenic mouse model IIb-tk previously developed in our laboratory (Tropel et al., 1997). In these mice, the promoter region of the murine CD41 (GPIIb) gene was used to target the expression of the thymidine kinase (tk) toxigene in cells specifically expressing the CD41 gene. The strength of the model resides in the fact that the depletion of the entire myelo-megakaryocytic lineage including stem cells/early progenitors by ganciclovir (GCV) administration could be inverted by discontinuing the treatment. A nearly full recovery of mature myelo-megakaryocytic cells is then observed within 3 days. To determine at the molecular level how BM cells could be regenerated in vivo following the eradication of all myeloid progenitors by the GCV treatment, microarray technology was applied. The strategy involved monitoring early transcriptional changes for the first 3-day of BM regeneration.
Project description:Standardized skin wounds were established surgically on mice and allowed to heal during a 15-day period. Expression of genes related to heparan sulfate biosynthesis was studied in wound bed and edges during the healing process. Keywords: Time course
