Project description:Tachykinins (TKs) and their receptors have been shown to be expressed in the mammalian ovary. However, the biological roles for ovarian TKs have yet to be verified. Ci-TK and Ci-TK-R, characterized from the protochordate (ascidian), Ciona intestinalis, are prototypes of vertebrate TKs and their receptors. In the present study, we show a novel biological function of TKs as an inducible factor for oocyte growth using C. intestinalis as a model animal. Immunostaining demonstrated the specific expression of Ci-TK-R in test cells residing in oocytes at the vitellogenic stage. DNA microarray and real-time PCR substantiated that Ci-TK induced gene expression of several proteases including cathepsin D, chymotrypsin, and carboxypeptidase B2 and functionally unidentified lectins or glycosidases in the ovary. The enzymatic activities of the above proteases in the ovary were also shown to be enhanced by Ci-TK. Of particular significance is that treatment of Ciona oocytes with Ci-TK resulted in progression of growth from the vitellogenic stage to the post-vitellogenic stage, which was completely blocked by a TK antagonist or protease inhibitors. These results led to the conclusion that Ci-TK enhances growth of the vitellogenic oocytes via up-regulation of gene expression and enzymatic activities of the proteases. Keywords: tachykinins, ovary

2008-08-19 | GSE8821 | GEO

Transcriptional profiling of macrophages in skin wounds

Project description:Transcriptional profiling of macrophages in skin wounds

| PRJNA761021 | ENA

Spatial Transcriptomic profiling of day2 wounds and intact tissues of Hgfac WT and KO mice

Project description:Wound healing is orchestrated by a spatial and temporal network of intercellular communication between epithelial cells, the stromal compartment, and the immune system. Our scRNA-seq analysis of Hgfac WT and KO wounds reveals that Aldh1a3 is downstream of the HGFAC system and Aldh1a3 is highly upregulated in wound-asociated epithelial (WAE) cells in responce to endoscopic wounding, suggesting WAE cells can potentially secrete retinoic acid into wound bed. To dissect the healing responce in the intestinal mucosa, we peformed spatial transcriptomics analysis.

2023-10-26 | GSE235742 | GEO

Single-Cell Transcriptomic Profiling of colonic wounds and intact tissues in Hgfac WT and KO mice

Project description:Wound healing is orchestrated by a spatial and temporal network of intercellular communication between epithelial cells, the stromal compartment, and the immune system. We found that Hgfac KO mice showed delayed wound healing in an endoscopic wound model. To dissect the celluar and molecular mechanisms of tissue healing, we collected tissues from day2 wounds or intact tissues from Hgfac WT and KO mice using a skin biopsy punch and dissociated cells for scRNA-seq analysis.

2023-10-26 | GSE235741 | GEO

Spatial Transcriptomic profiling of day2 wounds and intact tissues of Hgfac WT and KO mice

Project description:Spatial Transcriptomic profiling of day2 wounds and intact tissues of Hgfac WT and KO mice

| PRJNA987549 | ENA

Transcriptional profiling of wounds in WT mice and Cthrc1-/- mice

Project description:Collagen triple helix repeat containing 1 (CTHRC1) has been reported to be associated with tissue repair in response to injury. To investigate the role of CTHRC1 in the skin wound repair, we used next-generation sequencing (NGS) to clarify the changes of transcriptome in the mice skin wounds after Cthrc1 deletion.

2024-08-02 | GSE200576 | GEO

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