Project description:A comparison of different energetics based techniques for the characterization of young and old mice brain cell lysates. The techniques of stability of proteins from rates of oxidation (SPROX), thermal proteome profiling (TPP), Limited Proteolysis (LiP) and conventional expression level analyses were compared and the relative advantages and disadvantages are discussed.
Project description:Aging of the peripheral nervous system (PNS) is associated with structural and functional changes that lead to a reduction in regenerative capacity and the development of age-related peripheral neuropathy. Myelin is a central component in maintaining physiological peripheral nerve function, and differences in myelin maintenance, degradation, formation and clearance have been suggested to contribute to age-related PNS changes. In addition, recent proteomic studies have elucidated the complex composition of the total myelin proteome in health and its changes in neuropathy models. However, changes in the myelin proteome of peripheral nerves during ageing have not been investigated. Hence, the aim of this proteomics experiment was to define proteome changes in isolated myelin fractions during ageing, by investigating myelin proteome profiles from young (nerves from 2-3 month old mice) and old (nerves from 18 months old mice) nerves.
Project description:Young and old mice were first injected with bleomycin. At day 0, day 4 and day 14, the lungs were harvested and digested, and Lin-Epcam+ cells were isolated with FACS, single-cell RNA-seq was performed with the FACS-sorted cells.
Project description:We subjected old (21-22 month) and young (3-4 month) male C57BL/6 mice to 45 min transient oclusion of the middle cerebral artery and obtained the brain four days later. We obtained bodipy+ microglia from the ischemic brain tissue by FACS.
Project description:Aging is a key driver of cognitive decline and the predominant risk factor for several neurodegenerative diseases. Recent behavioral studies as well as structural and functional MRI data suggest that aging does not impact the brain in a uniform manner but follows region- and age-specific trajectories. Yet so far, quantitative analyses of the molecular dynamics in the aging brain have been limited to few regions at low temporal resolution. Here we performed single-nuclei sequencing (Nuc-seq) of hippocampus and caudate putamen isolated from young (3 months) and old (21 months) old mice.
Project description:Five populations from the regenerative region of the brain of young and old mice were sequenced to compare the type and degree of transcriptome-wide change with age between these different populations.
