Proteomics

Dataset Information

0

One-pot comparative analysis and characterization of young and old mice brain lysates


ABSTRACT: A comparison of different energetics based techniques for the characterization of young and old mice brain cell lysates. The techniques of stability of proteins from rates of oxidation (SPROX), thermal proteome profiling (TPP), Limited Proteolysis (LiP) and conventional expression level analyses were compared and the relative advantages and disadvantages are discussed.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Epithelial Cell

SUBMITTER: Morgan Bailey  

LAB HEAD: Michael C. Fitzgerald

PROVIDER: PXD036324 | Pride | 2024-05-23

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MouseTPP-OP_06-13-2020_01.msf Msf
MouseTPP-OP_06-13-2020_01.raw Raw
MouseTPP-OP_06-13-2020_02.raw Raw
MouseTPP-OP_06-13-2020_03.raw Raw
SPROX_enriched_1.msf Msf
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Publications

Comparative Analysis of Protein Folding Stability-Based Profiling Methods for Characterization of Biological Phenotypes.

Bailey Morgan A MA   Tang Yun Y   Park Hye-Jin HJ   Fitzgerald Michael C MC  

Journal of the American Society for Mass Spectrometry 20230220 3


Recently, a new suite of mass spectrometry-based proteomic methods has been developed that enables evaluation of protein folding stability on the proteomic scale. These methods utilize chemical and thermal denaturation approaches (SPROX and TPP, respectively) as well as proteolysis strategies (DARTS, LiP, and PP) to assess protein folding stability. The analytical capabilities of these technique have been well-established for protein target discovery applications. However, less is known about th  ...[more]

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