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Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE179065 | GEO | 2021/12/21

REPOSITORIES: GEO

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Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos.

Project description:The histone acetyltransferase Gcn5 is critical for gene expression and development. In Drosophila, Gcn5 is part of four complexes (SAGA, ATAC, CHAT and ADA) that are essential for fly viability and have key roles in regulating gene expression. Here, we show that although the SAGA, ADA and CHAT complexes play redundant roles in embryonic gene expression, the insect-specific CHAT complex uniquely regulates expression of a subset of developmental genes. We also identify a substantial decrease in histone acetylation in chiffon mutant embryos that exceeds that observed in Ada2b, suggesting broader roles for Chiffon in regulating histone acetylation outside of the Gcn5 complexes. The chiffon gene encodes two independent polypeptides that nucleate formation of either the CHAT or Dbf4-dependent kinase (DDK) complexes. DDK includes the cell cycle kinase Cdc7, which is necessary for maternally driven DNA replication in the embryo. We identify a temporal switch between the expression of these chiffon gene products during a short window during the early nuclear cycles in embryos that correlates with the onset of zygotic genome activation, suggesting a potential role for CHAT in this process. This article has an associated First Person interview with the first author of the paper.

| S-EPMC8917357 | biostudies-literature

iChiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [chiffon]

Project description:Purpose: The goal of this study was to identify the differential expressed genes in the Drosophila embryo when chiffon is disrupted.

2021-12-21 | GSE179061 | GEO

Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [ada2b]

Project description:Purpose: The goal of this study was to identify the differential expressed genes in the Drosophila embryo when ada2b is disrupted.

2021-12-21 | GSE179060 | GEO

Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [ChIP-seq]

Project description:Purpose: The goal of this study was to identify H3K14ac levels in chiffon mutant vs WT

2021-12-21 | GSE179062 | GEO

Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos

Project description:Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos

| PRJNA742034 | ENA

iChiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [chiffon]

Project description:iChiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [chiffon]

| PRJNA742042 | ENA

Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [ada2b]

Project description:Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [ada2b]

| PRJNA742041 | ENA

Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [ChIP-seq]

Project description:Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [ChIP-seq]

| PRJNA742043 | ENA

Dynamic pattern of histone H3 core acetylation in human early embryos.

Project description:After fertilization, highly differentiated sperm and oocyte are reprogrammed to totipotent embryo, which subsequently cleavages and develops into an individual through spatial-temporal differentiation. Histone modifications play critical roles to coordinate with other reprogramming events in early stages of embryogenesis. However, most of studies focus on modifications at N-terminus of histones, those at nucleosome core were not well understood. Here, we characterize the three key acetylation events in the histone H3 core, H3K56/K64/K122ac, in early human embryos. The three residues localize at DNA entry-exit position of the nucleosome. Globally, H3K56ac, H3K64ac and H3K122ac were detectable throughout preimplantation stages, with H3K64ac levels being relatively stronger and H3K122ac levels being much weaker. Besides, H3K56ac level had a peak at two-cell stage. Moreover, we found that LINEs also peak at two-cell stage, and H3K56ac was enriched at young LINE-1 in human ESCs, supporting that H3K56ac is an important driving force for young LINE-1 activation in human preimplantation embryos. Our results suggest that acetylation in the nucleosome core of histone H3 is dynamic and various during preimplantation development, and may drive diverse chromatin remodeling events in this developmental window.

| S-EPMC7513859 | biostudies-literature

Methylation-directed acetylation of histone H3 regulates developmental sensitivity to histone deacetylase inhibition.

Project description:Hydroxamate-based lysine deacetylase inhibitors (KDACis) are approved for clinical use against certain cancers. However, intrinsic and acquired resistance presents a major problem. Treatment of cells with hydroxamates such as trichostatin A (TSA) leads to rapid preferential acetylation of histone H3 already trimethylated on lysine 4 (H3K4me3), although the importance of this H3K4me3-directed acetylation in the biological consequences of KDACi treatment is not known. We address this utilizing Dictyostelium discoideum strains lacking H3K4me3 due to disruption of the gene encoding the Set1 methyltransferase or mutations in endogenous H3 genes. Loss of H3K4me3 confers resistance to TSA-induced developmental inhibition and delays accumulation of H3K9Ac and H3K14Ac. H3K4me3-directed H3Ac is mediated by Sgf29, a subunit of the SAGA acetyltransferase complex that interacts with H3K4me3 via a tandem tudor domain (TTD). We identify an Sgf29 orthologue in Dictyostelium with a TTD that specifically recognizes the H3K4me3 modification. Disruption of the gene encoding Sgf29 delays accumulation of H3K9Ac and abrogates H3K4me3-directed H3Ac. Either loss or overexpression of Sgf29 confers developmental resistance to TSA. Our results demonstrate that rapid acetylation of H3K4me3 histones regulates developmental sensitivity to TSA. Levels of H3K4me3 or Sgf29 will provide useful biomarkers for sensitivity to this class of chemotherapeutic drug.

| S-EPMC8053100 | biostudies-literature

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