Transcriptomics

Dataset Information

0

Transcriptomic and epigenomic analysis of germ-free and colitic mice


ABSTRACT: The gut microbiota influences host epigenetics by fermenting dietary fiber into butyrate. Although butyrate could promote histone acetylation by inhibiting histone deacetylases, it may also undergo oxidation to acetyl-CoA, a necessary cofactor for histone acetyltransferases. Here, we find that epithelial cells from germ-free mice harbor a loss of histone H4 acetylation across the genome except at promoter regions. Using stable isotope tracing in vivo with 13C-labeled fiber, we demonstrate that the microbiota supplies carbon for histone acetylation. Subsequent metabolomic profiling revealed hundreds of labeled molecules and supported a microbial contribution to host fatty acid metabolism, which declined in response to colitis and correlated with reduced expression of genes involved in fatty acid oxidation. These results illuminate the flow of carbon from the diet to the host via the microbiota, disruptions to which may affect energy homeostasis in the distal gut and contribute to the development of colitis.

ORGANISM(S): Mus musculus

PROVIDER: GSE179233 | GEO | 2022/12/12

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-12-11 | PXD026959 | Pride
2021-07-04 | MSV000087752 | MassIVE
2021-07-04 | MSV000087752 | GNPS
2017-10-30 | GSE104461 | GEO
2016-11-23 | GSE81117 | GEO
2016-11-23 | GSE81115 | GEO
2018-01-10 | PXD005004 | Pride
2016-11-21 | GSE79067 | GEO
2016-01-21 | ST000338 | MetabolomicsWorkbench
2023-11-21 | GSE235471 | GEO