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Transcriptomic analysis of in vitro induced germinal centre-like B cells and plasmablast differentiation

ABSTRACT: To elucidate molecular mechanisms that regulate the terminal differentiation of B cells into plasmablasts, we analysed how the B cell transcriptome changes during an in vitro culture system that mimics the germinal centre response (known as iGB culture). By culturing B cells on feeder cells expressing CD40L and B-cell activating factor BAFF in the presence of interleukin 4 and interleukin 21, this system allows class switch recombination to IgG1-producing cells and formation of CD138+ TACI+ B220low CD19int/low plasmablasts.

ORGANISM(S): Mus musculus

PROVIDER: GSE179281 | GEO | 2021/07/12

REPOSITORIES: GEO

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