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Single cell RNA-seq analysis of Mitochondrial ROS low CD34+ cells and Mitochondrial ROS high CD34+ cells

ABSTRACT: Ex vivo cultured human cord blood CD34+ cells were sorted as Mitochondrial ROS low cells(15%) and Mitochondiral ROS high cells (15%). These two populations were analyzed by single cell RNA-seq. Totally 18 cell clusters were identified by UMAP analysis.One of the clusters enriched with AVP, HLF,CD34, PROM1, MLLT3 and MSI2 was proved by in vivo transplantation experiments as the functional hematopoietic stem cell population. ADGRG1 combined with CD34 and PROM1 could be used to enrich functional hematopoietic stem cell upon ex vivo culture stress.

ORGANISM(S): Homo sapiens

PROVIDER: GSE179361 | GEO | 2021/09/30

REPOSITORIES: GEO

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