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Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC

ABSTRACT: Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC

PROVIDER: PRJNA517958 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8509460_1.fastq.gz	Fastqsanger.gz
	SRR8509460_2.fastq.gz	Fastqsanger.gz
	SRR8509470_1.fastq.gz	Fastqsanger.gz
	SRR8509470_2.fastq.gz	Fastqsanger.gz
	SRR8509480_1.fastq.gz	Fastqsanger.gz

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Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (ATAC-seq)

Project description:Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (ATAC-seq)

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Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (ChIP-seq)

Project description:Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (ChIP-seq)

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Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (RNA-seq)

Project description:Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (RNA-seq)

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Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (NG Capture-C)

Project description:Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (NG Capture-C)

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Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (RNA-seq)

Project description:Genome Wides Association Studies (GWAS) have identified tens of thousands of associations between human genetic variation and common disease. Despite the abundance of GWAS associations, functional identification and characterization of causative variants and effector genes remains a challenging prospect. Human erythropoiesis provides a highly tractable model system for the development of tools for GWAS analysis. We have developed a three-phase protocol for differentiation of peripheral CD34+ Haematopoietic Stem Cells (HSC) into mature enucleating erythrocytes. This protocol has been characterized for it’s the changes observed morphologically, immunologically (by FACS) and epigenetically (by ATAC-seq, ChIP-seq, and RNA-seq). We have used this protocol to study the effects of common GWAS variants affecting red blood cell traits, and more severe mutations causing Type-1 Congenital Dyserythropoietic Anemia (CDA-I).

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Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (ChIP-seq)

2023-01-21 | GSE125923 | GEO

Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (ATAC-seq)

2021-05-28 | GSE125922 | GEO

Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (NG Capture-C)

2023-01-21 | GSE125925 | GEO

Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-06-23 | GSE125926 | GEO

miRNA expression during UCB-CD34 cells erythropoiesis

Project description:We investigated the miRNA expression in ex vivo human erythroid cultures from umbilical cord blood (UCB)-derived CD34 cells. Hypothetically, the decline of certain miRNAs may promote erythropoiesis by unblocking expression of key functional proteins while the up-regulation of other miRNAs may block commitment to non-erythroid lineages. Therefore, discovering the patterns and sequence of miRNA expression during hematopoietic differentiation will provide insights about the functional roles of these tiny noncoding genes. Keywords: miRNA expression profiling

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