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The skin transcriptome analysis of yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

ABSTRACT: The skin transcriptomes were examined by high-throughput sequencing at control group and 16 h transport group. A total of 1577 genes were differentially expressed, including 892 up-regulated and 685 down-regulated. It is interesting to note that the qPCR analysis further confirmed that the skin's immune response to transport stress was activated via Toll-like receptors (TLRs) and NOD-like receptors (NLRs) in skin: the tlr9, mfn2 and ikbke were significantly up-regulated and nfkbia, map3k7cl were significantly down-regulated. Overall results suggested that transport stress. The overall results suggest that the skin responds to transport stress by regulating mucus secretion and activating the immune signal pathway. This study will help to optimize the transport conditions for cultured hybrid yellow catfish.

ORGANISM(S): Tachysurus fulvidraco x Tachysurus vachelli

PROVIDER: GSE179444 | GEO | 2021/07/07

REPOSITORIES: GEO

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Project description:The transport of live fish is a necessary step for commercial production. The skin of teleost fish is the first non-specific immune barrier against exogenous stimuli, and it plays an important protective role under transport stress. Thus, the aim of this study was to explore the skin responses to transport stress in hybrid yellow catfish (Tachysurus fulvidraco♀ × Pseudobagrus vachellii♂) through transcriptome and biochemical analyses. Water samples were collected during a simulated transport treatment. Biochemical indexes and/or gene expression in blood, skin, and mucus in fish in control groups and transport-stress groups (0 h, 2 h, 4 h, 8 h, 16 h) were assayed. The levels of total ammonia–nitrogen and nitrite–nitrogen in the water increased with increasing transport time. Comparison of skin transcriptomes between the control group and the group subjected to 16 h of transport revealed 1547 differentially expressed genes (868 up-regulated and 679 down-regulated). The results of the transcriptome analysis were validated by analyses of the expression levels of selected genes by qRT-PCR. The results indicated that the toll-like receptors and nod-like receptors signaling pathways mediate the skin’s immune response to transport stress: tlr9, mfn2, and ikbke were significantly up-regulated and nfkbia and map3k7cl were significantly down-regulated under transport stress. With increasing transport time, lysozyme activity and the immunoglobulin M content in skin mucus first increased and then decreased. The number of mucous cells peaked at 8 h of transport stress, and then decreased. The mucus cells changed from types II and IV to types I, II, III, and IV. The amounts of red and white blood cells and the levels of hemoglobin and hematocrit first increased and then decreased during 16 h of transport stress. Together, the results showed that the skin responds to transport stress by activating the immune signaling pathway and regulating mucus secretion. These findings have important biological significance for selecting strains that tolerate transport, as well as economic significance for optimizing the transport conditions for scaleless fish.

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The skin transcriptome analysis of yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

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