Dataset Information

The gills transcriptome analysis of yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

ABSTRACT: Transport stress is a big threat to most teleost fish during production practice, causing mass loss to the aquaculture industry. Fish gills are mucosa-associated lymphoid tissue that directly contacted with water, which is the good choice to study the mechanism of transport stress. Gills transcriptome results revealed 1551 differentially expressed genes (744 up-regulated and 807 down- regulated) between the control and 16h transportation groups. The top 10 enriched KEGG pathways are all immune-related. After qRT-PCR validation, we found that the toll-like receptors and nod-like receptors signaling pathways mediate the gill’s immune response to transport stress: tlr5, tnfɑ, hsp90ɑ, il-1ß and map2k4 were significantly up-regulated and arrdc2 was significantly down-regulated under transport stress. These results suggested that the transport stress altered innate immunity responses and induced metabolic changes in gills of hybrid yellow catfish. In conclusion, the results provide new perspectives on immune response in yellow catfish against transport stress and provide the possibility of mining resistance genes for future optimize transportation plan.

ORGANISM(S): Tachysurus fulvidraco x Tachysurus vachelli

PROVIDER: GSE192657 | GEO | 2021/12/29

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Live fish transport often results in mass mortality, but the underlying mechanisms are not well known. Largemouth bass (LMB, Micropterus salmoides) juveniles were subjected to 4-hour transportation followed by 5 days of recovery. Mortality (33.61 ± 10.58 %) of the transported fish mainly occurred between 2 and 3 days of recovery. Except for the dead fish, the survivors demonstrating abnormal symptoms were considered more sensitive to the transport stress than the normal ones. Liver of the abnormal fish had significantly (p < 0.05) lower glutathione (GSH) content and superoxide dismutase (SOD) activity, and irregular cellular morphology. The liver and spleen collected before transportation (T0), at the end of transportation (Te), after 1 day of recovery (R1d), and after 3, 4 and 5 days of recovery (R3d, R4d and R5d, from both the normal and abnormal fish) were subjected to RNA-sequencing. Time series gene expression data of the T0, Te, R1d, R3d-R5d (from the normal fish) samples were analyzed to explore transcriptional responses elicited by the transport stress. Transportation-activated transcriptional regulations could be classified as immediate response (Te), and early (R1d) and late recovery (R3d to R5d) phase in both tissues. High tissue specificity was found for functional enrichments of the up-regulated genes. The up-regulated genes of the liver are highly enriched in cholesterol, sterol and steroid biosynthesis, while those of the spleen are primarily associated with hemopoiesis and immune response. However, large numbers of functional enrichments were shared by the down-regulated genes of the liver and spleen, especially during the recovery phase. Representatives of the depressed processes included neurogenesis, angiogenesis and cell migration, etc. Moreover, large numbers of differentially expressed genes (DEGs) were identified between the normal and abnormal fish. Functional enrichment analyses for the DEGs revealed that failing to terminate the stress responses in the spleen and over depression of the physiological roles of the liver are critical for stress susceptibility of the abnormal fish. Furthermore, an array of core genes associated with stress susceptibility were identified. Our data provide novel insights into the genetic factors underlying susceptibility of LMB to the transport stress.

Project description:Background: Yellow catfish (Pelteobagrus fulvidraco) is one of the important aquaculture species in China. In recent years, due to the high breeding density, the increasing frequency of feeding, and the excessive addition of feed fat, the excessive deposition of body fat in cultured yellow catfish has become more frequent. MicroRNAs (miRNAs) are an important gene expression regulatory signal molecule that regulates liver fat synthesis and transport and play an important role in fat deposition. However, there is a little research on the mechanism of fatty liver caused by excessive deposition of liver fat. Results: After 60 days of high-fat stress, the growth and feed conversion rate of hybrid yellow catfish (Pelteobagrus fuIvidraco♀×P. vachelli♂) were significantly inhibited, and hepatosomatic index, viscerosomatic index, hepatic triglyceride and cholesterol, and red lipid droplets in liver tissues were increased. Through high-throughput sequencing, we constructed miRNA libraries of high-fat stress at 60d, identified 346 conserved miRNAs and 410 novel miRNAs, among which 13 differentially expressed miRNAs were screened between high-fat diet group and normal-fat diet group. Also, we constructed mRNA transcriptome libraries after high-fat stress. Potential target genes for differentially expressed miRNAs were identified by bioinformatics analysis. Seven miRNA-mRNA pairs were screened. The expression of differential miRNA and mRNA and potential binding sites were analyzed by qRT-PCR and dual luciferase assay. Hybrid yellow catfish could promote the oxidative degradation of liver glucose, reduce fatty acid peroxidation, regulate antioxidant enzyme activity and response of immune and inflammatory to relieve fat deposition and liver stress. Conclusions: The disorders of fat metabolism in liver not only result in feed wastage, increase metabolic burden of yellow catfish, but also cause immune function damage, resulting in a variety of nutritional diseases. The development of this study is to understand the molecular mechanism of hepatic fat deposition in yellow catfish. It has important biological significance for improving protection of liver against stress and healthy culture.

Dataset Information

The gills transcriptome analysis of yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets