Mammalian UPF3A and UPF3B activate NMD independently of their EJC binding
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ABSTRACT: Nonsense-mediated mRNA decay (NMD) can occur independently of some NMD factors such as UPF3B. We have performed total RNA-Seq in HCT116 cells under NMD factor knockout (with CRISPR-Cas9) and/or knockdown (with siRNA transfection) conditions to identify mRNA substrates regulated by different NMD factors. We have also performed RIPiT-Seq to identify the footprints of three compositionally distinct EJCs that contain the following pairs of proteins: MAGOH-EIF4A3, UPF3B-EIF4A3 or CASC3-EIF4A3 in WT HCT116 cells.
ORGANISM(S): Homo sapiens
PROVIDER: GSE179843 | GEO | 2022/04/23
REPOSITORIES: GEO
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