Single cell gene expression profiling of cell lines and murine peripheral blood mononuclear cells (PBMCs)
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ABSTRACT: To demonstrate the efficacy of our developed Automated Live-imaging and cell Picking System for downstream RNA sequencing (ALPS&RNA-seq), we isolated single cells of three cell lines (murine T cell, murine leukemia cell, and murine hematopoietic progenitor cell) and of murine peripheral blood mononuclear cells (PBMCs). The detemined profiles of the three cell lines were different, and the profiles of the PBMSc showed three subpopulations which were determined as CD4+ T cells, CD8+ T cells, and B cells based on the marker genes.
ORGANISM(S): Mus musculus
PROVIDER: GSE179943 | GEO | 2022/12/15
REPOSITORIES: GEO
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