Project description:The cloaca is a transient structure that forms in the terminal hindgut giving rise to the rectum dorsally and the urogenital sinus ventrally. Similarly, human hindgut cultures derived from human pluripotent stem cells generate human colonic organoids (HCOs) which also contain co-developing urothelial tissue. In this study, our goal was to identify pathways involved in cloacal patterning and apply this to human hindgut cultures. RNA-seq data comparing dorsal versus ventral cloaca in e10.5 mice revealed that Wnt signaling was elevated in the ventral versus dorsal cloaca. Inhibition of WNT signaling in hindgut cultures biased their differentiation towards a colorectal fate. WNT activation biased differentiation towards a urothelial fate, giving rise to human urothelial organoids (HUOs). HUOs contained cell types present in human urothelial tissues. Based on our results, we propose a mechanism whereby WNT signaling patterns the ventral cloaca, prior to cloacal septation, to give rise to the urogenital sinus.
Project description:Transcriptional profiling of Bmi1 mutant dental epithelia including the stem cell compartment to determine which genes are upregulated in response to loss of Bmi1. Two condition experiment: dental epithelia homozygous null for Bmi1 and WT dental epithelia. 4 replicates each
Project description:The goal of this study was to compare cell composition and gene expression of different cell types in healthy primary human airway epithelia cultured at the air-liquid interface
Project description:The goal of this study was to compare cell composition, gene expression, and infectivity of different cell types in human airway epithelia following exposure to measles virus. Samples included control epithelia exposed to a mock infection and measles-virus-exposed epithelia that were sorted according to detection of green fluorescent protein (GFP) prior to library preparation and sequencing.
Project description:Transcriptional profiling of Bmi1 mutant dental epithelia including the stem cell compartment to determine which genes are upregulated in response to loss of Bmi1.
Project description:The goal of this study was to examine changes to gene expression induced by IL-13 treatment of air-liquid interface cultures of healthy primary human airway epithelia over time.
Project description:The goal of this study was to examine changes in cell composition and gene expression with IL-13 treatment over time within different cell types from air-liquid interface cultures from healthy primary human airway epithelia.
