Transcriptomics

Dataset Information

0

Analysis of gene expression levels in BBF2H7-/- chondrocytes


ABSTRACT: BBF2H7 (BBF2 human homolog on chromosome 7), an ER-resident basic leucine zipper transcription factor, is activated in response to ER stress and abundantly expresses in chondrocytes. While BBF2H7 is widely expressed in many tissues and organs, the most intense signals were detected in the proliferating zone of the cartilage. We compared gene expressions in primary cultured chondrocytes prepared from rib cartilage between WT and BBF2H7-/- mice at E18.5. Primary cultured chondrocytes were prepared from E18.5 rib cartilage of WT and BBF2H7-/- mice. Chondrocytes were isolated using 0.2% collagenase D (Roche) after adherent connective tissue was removed by 0.2% trypsin (Sigma) and collagenase pretreatment. Isolated chondrocytes were maintained in α-MEM (Gibco) supplemented with 10% FCS and 50 µg/mL ascorbic acid. Adenovirus vectors expressing the mouse p60 BBF2H7 (1-377 aa, BBF-N) were constructed with the AdenoX Expression system (Clontech), according to the manufacturer’s protocol. The cells were infected with adenoviruses 30 h before analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE18052 | GEO | 2009/09/11

SECONDARY ACCESSION(S): PRJNA119309

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2010-05-05 | E-GEOD-18052 | biostudies-arrayexpress
2018-08-01 | GSE102292 | GEO
2021-03-14 | E-MTAB-10258 | biostudies-arrayexpress
2010-10-15 | E-GEOD-18992 | biostudies-arrayexpress
2010-10-15 | E-GEOD-18991 | biostudies-arrayexpress
2023-09-20 | GSE199193 | GEO
2015-09-15 | E-GEOD-67132 | biostudies-arrayexpress
2010-01-01 | E-GEOD-16007 | biostudies-arrayexpress
2016-07-03 | E-GEOD-68038 | biostudies-arrayexpress
2015-09-15 | GSE67132 | GEO