Transcriptomics of THP-1 macrophages after treatment with NLRP3-induced EVs

ABSTRACT: Inflammasome activation in macrophages induces the release of EVs, however, the effect of these inflammasome-induced EVs on recipient cells is poorly characterized. To characterize the effect EVs released upon LPS + nigericin stimulation, we performed 3' sequencing on the recipient cells (NLRP3 KO THP-1 macrophages and NLRP3 KO THP-1 macrophages that have been reconstituted with NLRP3 to resemble the WT). As controls, RNA isolated from EVs themselves or LPS- or nigericin-treated cells were subjected to 3' sequencing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE180707 | GEO | 2021/07/26

REPOSITORIES: GEO