Genome-wide CRISPR screen to identfy immune evasion genes in pancreatic cancer
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ABSTRACT: Here we systematically dissected PDA intrinsic mechanisms of immune evasion by in vitro and in vivo CRISPR screening. Beyond the conserved set of genes regulating anti-cancer immunity we identified Rnf31 and Vps4b as essential factors required for escaping CD8+ T cell-killing. While the absence of Rnf31 induced sensitivity to T cell killing through TNF-mediated apoptosis in murine cancer cells and human PDA organoids, loss of Vps4b abrogated functional autophagy, resulting in accumulation of CD8+ T cell-derived granzyme B and subsequent tumor cell lysis.
ORGANISM(S): Mus musculus
PROVIDER: GSE180834 | GEO | 2021/07/27
REPOSITORIES: GEO
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