Rat Adipose Tissue
Ontology highlight
ABSTRACT:
ORGANISM(S): Rattus norvegicus
PROVIDER: GSE1813 | GEO | 2004/11/30
SECONDARY ACCESSION(S): PRJNA90297
REPOSITORIES: GEO
Similar Datasets
Project description:Male 130 day old rat adipose tissue from control and obese rats
2008-06-11 | E-GEOD-1813 | biostudies-arrayexpress
Project description:Obesity is characterised by increased adipocyte size and number. Analysis of altered gene expression gives better understading about the mechanisms involved/alterted in the development of obesity in this new obese rat model. We used Microarrays to delinate the alted gene expression in adipose tissue of WNIN/Ob obese rats Retroperitioneal adipose tissue was collected from 4 month old WNIN/Ob lean and obese rats (n=2 per phenotype) for RNA extraction and hybridization on Affymatrix Rat gene 1ST arrays.
2014-06-18 | E-GEOD-58575 | biostudies-arrayexpress
Project description:Obesity is characterised by increased adipocyte size and number. Analysis of altered gene expression gives better understading about the mechanisms involved/alterted in the development of obesity in this new obese rat model. We used Microarrays to delinate the alted gene expression in adipose tissue of WNIN/Ob obese rats
2014-06-18 | GSE58575 | GEO
Project description:MicroRNAs (miRNAs) are non-coding RNA molecules involved in post-transcriptional control of gene expression of a wide number of genes, including those involved in glucose homeostasis. Type 2 diabetes (T2D) is characterized by hyperglycaemia and defects in insulin secretion and action at target tissues. Using a miRNA microarray platform, we sought to establish differences in miRNA expression in two insulin-target tissues (liver and adipose tissue) from seven-month-old spontaneously diabetic (Goto-Kakizaki [GK]) and non-diabetic (Brown-Norway [BN]) rats. MiRNA data were integrated with gene expression data from the same rats to investigate how differentially expressed miRNAs affected the expression of their predicted target genes. Two-colour experiment comparing GK and BN rat strains for two different tissues. Biological replicates: 4 GK and 4 BN rats; adipose tissue and liver extracted from each rat. Two samples were hybridised to each array (one of each strain, same tissue)
2010-05-16 | E-GEOD-17058 | biostudies-arrayexpress
Project description:Thyroid hormones (THs) influence adipose tissue development and metabolism. They regulate both adipocyte proliferation and differentiation and, as they increase the metabolic rate, may be helpful for obesity treatment. However, due to their cardiotoxic effects, like tachycardia and arrhythmia, their use is limited to hypothyroid obese patients. Interestingly, some TH metabolites have been recently shown to possess the same beneficial metabolic effects as THs without any negative effect. The biogenic amine 3-Iodothyronamine (T1AM), for example, affects carbohydrate and lipid metabolism without undesirable side effects. In mice T1AM exhibits cardiac effects opposite to those associated with thyroid hormones, like bradycardia, and, in isolated working rat hearts, produces a rapid, reversible, dose-dependent decrease in cardiac output, aortic pressure and coronary flow. Intraperitoneal injections of T1AM, moreover, induce reduction of RQ from 0.9 to 0.7, both in mice and Djungarian hamsters, indicating that carbohydrate utilisation is reduced and energy requirements are covered by lipid consumption. Ketone bodies in the urine and the significant loss of body fat mass confirm that lipids are predominantly used to cover the energy requirements in response to T1AM administration. The molecular mechanisms by which T1AM favors the lipid rather than glucose catabolism are not known, but it is possible to envisage changes in gene expression, given the delayed and long-lasting phenotypic effects. The complete RQ shift, for example, is reached 4.5 h after the T1AM injection and persists at least for 24 h. In this paper we analyzed the gene expression profiles in subcutaneous adipose tissue and in liver of eight rats chronically treated with T1AM as compared with eight untreated rats. Many genes linked to lipid metabolism, adipogenesis and angiogenesis appeared modulated by T1AM, thus contributing to explain the T1AM phenotypic effects observed in rodents. Furthermore, T1AM influenced the expression of several genes relating to lipoprotein metabolism that provide new insights on T1AM mechanism of action, like, for example, the regulation of cholesterol homeostasis.
2014-09-08 | E-MTAB-2177 | biostudies-arrayexpress
Project description:White adipose tissue is a central place to energy storage and a major endocrine organ. However, adipose molecular mechanisms have been poorly studied during prolonged fasting. To fill this gap, the aim of this study was to decipher proteomic regulations in rat adipose tissue during phase 2 (lipid mobilization) and phase 3 (protein catabolism) of prolonged fasting compared to the fed state. Specific responses reflecting adipose tissue inflammation, increased fibrinolysis and a possible protein catabolism-related energy saving mechanism were recorded during phase 3. Differences between internal and subcutaneous adipose tissues were essentially related to lipid metabolism, the response to oxidative stress and energy production. These data thus provide a molecular basis of adipose tissue responses according to the fasting stage.
2021-02-24 | PXD010902 | Pride
Project description:The genetic control of kidney and white adipose tissue transcriptomic regulation was investigated in Goto-Kakizaki (GK), Brown Norway and hybrids of an F2cross between GK and BN rats using Illumina Beadchips. Expression data was determined in 5 months old rats.
2014-01-20 | E-MTAB-969 | biostudies-arrayexpress
Project description:The goal of this study is to reveal unique features of adipose Tregs and to study mechanisms underlying how mediator Med23 regulate adipose Treg function in aged mice. Therefore, mRNA profiles of LN Tregs (from 4-month old Foxp3Cre mice) and adipose Tregs (from 4-month old Foxp3Cre, 4-month old obese Foxp3Cre, 10-month old Foxp3Cre and 10-month old Med23fl/fl;Foxp3Cre mice) were generated by deep sequencing, single experiment, using Illumina HiSeq2000. We compared transcriptome features between lymph node-derived Tregs and adipose Tregs from 4-month old lean or obese mice. Using unbiased comparative gene expression analyses, we found adipose Tregs display an up-signature of Insr (Insulin receptor) and Hif1a, while Pparg acts as a positive control. We next compared gene expression profiles of adipose Tregs from 10-month old Med23fl/fl;Foxp3Cre (MKO) and Foxp3Cre (WT) mice. adipose Med23-ΔTreg cells display impaired transcription of Pparg and Il1rl1 (ST2), and they simultaneously acquire the expression of Nt5e (CD73), while Entpd1 (CD39) expression was not dramatically altered. Our studies implicate protective roles of CD73hi adipose Tregs and offer new therapeutic strategies against age-associated metabolic syndrome.
2021-07-15 | GSE124721 | GEO
Project description:To investigate the proteomic profiles of paired subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) samples, as well as their correlations with clinical traits in severely obese patients, and to identify potential serum protein markers associated with tissue expression or metabolic states.
2023-10-16 | PXD041721 | Pride
Project description:Type 2 diabetes (T2D) is characterized by hyperglycaemia and defects in insulin secretion and action at target tissues. Using the Illumina RatRef-12 v1.0 array, gene expression was assessed in two insulin-target tissues (liver and adipose tissue) from seven-month-old spontaneously diabetic (Goto-Kakizaki [GK]) and non-diabetic (Brown-Norway [BN]) rats. This study was performed in parallel with miRNA expression profiling of the same rats.
2009-07-14 | GSE17059 | GEO