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Transcriptomes of miR-290-295 KO mESCs treated with siPOOLs against the transcription factor Tfap4

ABSTRACT: In order to identify direct microRNA targets in mouse embryonic stem cells (mESCs), we knocked out microRNA effector genes, profiled their transcriptomes and integrated the data with additional published OMICs data sets. To validate the analysis, we generated miR-290-295 KO mutant cell lines, assessed the transcriptome profile and compared the observed misregulation to the predictions of the analysis. We identified the transcription factor Tfap4 as novel potential direct and functional target of miR-290-295. To assess the role of Tfap4, we rescued its expression in the miR-290-295 KO cell line using siPOOLs and profiled the transcriptome.

ORGANISM(S): Mus musculus

PROVIDER: GSE181393 | GEO | 2022/07/15

REPOSITORIES: GEO

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Transcriptomes of miR-290-295 KO mESCs treated with siPOOLs for three different transcription factors and a negative control

Project description:In order to identify direct microRNA targets in mouse embryonic stem cells (mESCs), we generated miR-290-295 KO mutant cell lines, assessed their transcriptomes. We identified novel transcription factors (TF) as potential direct and functional targets of miR-290-295. To assess the role of these TFs, we rescued their expression in the miR-290-295 KO cell line using siPOOLs and profiled their transcriptomes.

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