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Analyses of translatome combined with transcriptome in Arabidopsis shoot and root reveals new players responding to magnesium deficiency.

ABSTRACT: Translational control of gene expression, including recruitment of ribosome to mRNA, is particularly important for stress responses. Translating Ribosome Affinity Purification (TRAP)-based RNA-sequencing offers a tool to study ribosome-associated mRNAs, which better proxies protein levels via the levels of transcripts to be translated into proteins. Magnesium (Mg) greatly inhibits plant growth and affects crop productivity. To identify new players for better understanding plants in response to Mg deficiency at the translational level, we combined the transcriptome with translatome analyses. We revealed that 26 previously unreported Mg responsive genes, which were only regulated at the translational level, not transcriptional level at an early stage upon Mg deficiency. Among six mutants analyzed, we found that mutants of the transcriptional factor of ELONGATED HYPOCOTYL 5 (HY5), H+/cation exchanger 1 and 3 and UBIQUITIN 14 exhibited early chlorosis phenotype under Mg deficiency. UBIQUITIN 14 is the first UBIQUITIN protein reported to be functionally involved in ion homeostasis. Overall, our study demonstrates that there is a strong correlation between ribosome-associated mRNA abundance and mutant phenotypes, strongly supporting that TRAP-seq combined with RNA-seq followed by phenotype screening could serve as a valuable tool to identify novel players during stress responses.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE181536 | GEO | 2022/01/30

REPOSITORIES: GEO

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