Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Real-time quantitative PCR analysis of mouse cerebellum oligodendrocytes

ABSTRACT: Oligodendrocytes were aspirated after electrophysiology recordings for qPCR analysis from cerebellum slices

ORGANISM(S): synthetic construct Mus musculus Homo sapiens

PROVIDER: GSE181631 | GEO | 2021/08/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Real-time quantitative PCR analysis of baboon cerebellum oligodendrocytes

Project description:Oligodendrocytes were aspirated after electrophysiology recordings for qPCR analysis from cerebellum slices

2021-08-08 | GSE181633 | GEO

Real-time quantitative PCR analysis of mouse brainstem oligodendrocytes

Project description:Oligodendrocytes were aspirated after electrophysiology recordings for qPCR analysis from cerebellum slices

2021-08-08 | GSE181630 | GEO

Real-time quantitative PCR analysis of mouse brainstem oligodendrocytes, mouse cerebellum oligodendrocytes and baboon cerebellum oligodendrocytes

Project description:Real-time quantitative PCR analysis of mouse brainstem oligodendrocytes, mouse cerebellum oligodendrocytes and baboon cerebellum oligodendrocytes

| PRJNA752773 | ENA

Real-time quantitative PCR analysis of mouse cerebellum oligodendrocytes

Project description:Real-time quantitative PCR analysis of mouse cerebellum oligodendrocytes

| PRJNA752775 | ENA

Real-time quantitative PCR analysis of baboon cerebellum oligodendrocytes

Project description:Real-time quantitative PCR analysis of baboon cerebellum oligodendrocytes

| PRJNA752776 | ENA

Real-time quantitative PCR analysis of mouse brainstem oligodendrocytes, mouse cerebellum oligodendrocytes and baboon cerebellum oligodendrocytes

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-08-08 | GSE181634 | GEO

Real-time quantitative PCR analysis of mouse brainstem oligodendrocytes

Project description:Real-time quantitative PCR analysis of mouse brainstem oligodendrocytes

| PRJNA752774 | ENA

Single-Cell Transcriptomes Reveal Molecular Specializations of Neuronal Cell Types in the Developing Cerebellum

Project description:We analyzed transcriptome profiles of 21,119 single cells of the postnatal mouse cerebellum and identified 8 main cell clusters (Granule precursors, Granule cells, Astrocytes, Interneurons, Oligodendrocytes, Microglia, Purkinje cells, Endothelial cells).

2019-03-12 | GSE122357 | GEO

snATAC-seq of the gray short-tailed opossum cerebellum in two developmental stages

Project description:This study used droplet-based snATAC-seq to profile the chromatin accessibility landscape of 19,204 nuclei in the opossum (Monodelphis domestical) cerebellum across two developmental stages (postnatal day 21 and adult). The study included two biological replicates per stage, one from each sex, and an additional adult sample enriched for white matter. Cerebelli were dissected in two halves, nuclei were extracted from one half and profiled using 10x single-cell ATAC reagent kit (v1.1) and a Chromium controller. The white matter enriched sample was dissected from coronal cerebellum slices. Libraries were sequenced using paired-reads on Illumina NextSeq 550 and initial data processing was performed using Cellranger ATAC (1.1).

2021-09-07 | E-MTAB-10533 | biostudies-arrayexpress

Transcription profiling of rat perineuronal A2B5/OTMP+ oligodendrocytes

Project description:Oligodendrocytes are cells from the central nervous system that can be grouped into precursors, myelin-forming, and non-myelinating perineuronal. The function of perineuronal oligodendrocytes is unknown; it was suggested that they can ensheath denuded axons. We tested this hypothesis. Using cell-specific tags, microarray technology and bioinformatics tools to identify gene expression differences between these subpopulations allowed us to capture the genetic signature of perineuronal oligodendrocytes. Here we report that perineuronal oligodendrocytes are configured for a dual role. As perineuronal, they integrate a repertoire of transcripts designed to create a cell with its own physiological agenda. But they maintain a reservoir of untranslated transcripts encoding the major myelin proteins for â we speculate â a pathological eventuality. We posit that the signature molecules PDGFR-Î±Î², cytokine PDGF-CC, and the transcription factor Pea3 used â among others - to define the non-myelinating phenotype, may be critical for mounting a myelinating programme during demyelination. Harnessing this capability is of therapeutic value for diseases such as multiple sclerosis. This is the first molecular characterization of perineuronal oligodendrocytes. Experiment Overall Design: Flow Cytometry was used to isolate A2B5/OTMP+ oligodendrocytes from postnatal day 7 rat brain. 4 bioloical replicates were obtained. This data can be compared to the previously submitted oligodendrocyte expression data (GSE5940).

2008-06-18 | E-GEOD-11277 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data