Dataset Information

A multi-omics approach incorporating single-cell RNASequencing reveals murine Leishmania major infection drives a proinflammatory signature alongside a signature of decreased ribosomal biogenesis.

ABSTRACT: RNA-seq analysis was performed in ear samples from naïve control and L. major infected mice. Using scRNASeq, we investigated the transcriptomic changes within individual cell types present in leishmanial lesion. Transcriptomic analysis through scRNASeq revealed 35 distinct cell populations which include both resident and recruited cell types following L. major infection. In addition, our scRNASeq analysis confirms a significant recruitment of various immune cell types such as macrophages, inflammtory monocytes, neutrophils, dendritic cells, CD4 and CD8 T cells in the infected ears that are seen at higher frequencies compared to naive controls. In overall, our scRNASeq data revealed that DEGs within each immune cell type driving a proinflammtory state during L. major infection, alongside a signature of decreased ribosomal biogenesis.

ORGANISM(S): Mus musculus

PROVIDER: GSE181720 | GEO | 2021/08/12

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:The circadian system produces ~24-hr oscillations in behavioral and physiological processes to ensure that they occur at optimal times of day and in the correct temporal order. At its core, the circadian system is composed of dedicated central clock neurons that keep time through a cell-autonomous molecular clock. To produce rhythmic behaviors, time-of-day information generated by clock neurons must be transmitted across output pathways to regulate the downstream neuronal populations that control the relevant behaviors. An understanding of the manner through which the circadian system enacts behavioral rhythms therefore requires the identification of the cells and molecules that make up the output pathways. To that end, we recently characterized the Drosophila pars intercerebralis (PI) as a major circadian output center that lies downstream of central clock neurons in a circuit controlling rest:activity rhythms. We have conducted single-cell RNA sequencing (scRNAseq) to identify potential circadian output genes expressed by PI cells, and used cell-specific RNA interference (RNAi) to knock down expression of ~40 of these candidate genes selectively within subsets of PI cells. We demonstrate that knockdown of the slowpoke (slo) potassium channel in PI cells reliably decreases circadian rest:activity rhythm strength. Interestingly, slo mutants have previously been shown to have aberrant rest:activity rhythms, in part due to a necessary function of slo within central clock cells. However, rescue of slo in all clock cells does not fully reestablish behavioral rhythms, indicating that expression in non-clock neurons is also necessary. Our results demonstrate that slo exerts its effects in multiple components of the circadian circuit, including PI output cells in addition to clock neurons, and we hypothesize that it does so by contributing to the generation of daily neuronal activity rhythms that allow for the propagation of circadian information throughout output circuits.

Dataset Information

A multi-omics approach incorporating single-cell RNASequencing reveals murine Leishmania major infection drives a proinflammatory signature alongside a signature of decreased ribosomal biogenesis.

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets