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Elucidating the stromal expression pattern in response to tumor-derived Shh

ABSTRACT: Most tumors of the upper gastrointestinal tract are known to depend on an excessive expression of Shh. It was recently discovered that this Shh does not signal on the tumor cells, but rather the stromal cells that in turn produce an unknown set of reciprocal signals that act as growth- or survival cues for the tumor cells. This sequencing effort aims to identify the reciprocal signals.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE18245 | GEO | 2010/09/24

SECONDARY ACCESSION(S): PRJNA119635

REPOSITORIES: GEO

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Elucidating the stromal expression pattern in response to tumor-derived Shh

Project description:Most tumors of the upper gastrointestinal tract are known to depend on an excessive expression of Shh. It was recently discovered that this Shh does not signal on the tumor cells, but rather the stromal cells that in turn produce an unknown set of reciprocal signals that act as growth- or survival cues for the tumor cells. This sequencing effort aims to identify the reciprocal signals. Mouse fibroblasts and Shh-expressing human pancreatic adenocarcinoma cells were either grown alone, or in a non-adherent coculture system. To be able to assess the effect of Shh on fibroblast gene expression (being the reciprocal signal), 5E1 blocking antibody was added to the cells and after 5 days, RNA was isolated. n=1

2010-09-24 | E-GEOD-18245 | biostudies-arrayexpress

Elucidating the stromal expression pattern in response to tumor-derived Shh

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HHIP-AS1 lncRNA promotes tumorigenicity in human SHH-driven brain tumors

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Elucidating the mechanism underlying prostate turmorigenesis mediated by AR in Shh-responsive cells

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Expression data from adult Shh-responding astroglia cells in mice

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Expression data from adult Shh-responding astroglia cells in mice

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