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Expression profiles of colon macrophages from macrophage-specific E4BP4 transgenic mouse [ColonMac_RNA-seq]

ABSTRACT: Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the effects of macrophage-specific E4BP4 overexpression under c-fms gene promoter on colon macrophages during the recovery phase of Dextran Sodium Sulfate (DSS)-induced colitis. Methods: We generated transgenic mice (TG) with macrophage-specific E4BP4 overexpression. Colon macrophages were isolated at Zeitgeber Time (ZT) 0 from TG mice and WT littermates and total RNA was extracted. Colon macrophage RNA profiles were generated by deep sequencing for two groups with three mouse samples each. Results: There were significant differences between TG and WT mice. Conclusions: Colon macrophages from the E4BP4 transgenic mice during the recovery phase of DSS-induced colitis might be altered at the transcription level.

ORGANISM(S): Mus musculus

PROVIDER: GSE183075 | GEO | 2024/03/31

REPOSITORIES: GEO

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Expression profiles of CD45+ cells in colon lamina propria from macrophage-specific E4BP4 transgenic mouse

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Expression data from untreated and DSS-treated Adamts12 WT and KO mice

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Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the effects of liver-specific E4BP4 overexpression under mouse albumin promoter on the liver glucose and lipid metabolism. Methods: We generated transgenic mice (TG) with liver-specific E4BP4 overexpression, and harvested two livers with one TG mouse each. After cervical dislocation, mouse liver was perfused with a buffer containing Hanks' balanced salt solution, and cross-inked for 30 minutes with 2mM DSG and for 10 minutes with 1% formaldehyde by perfusion, followed by frozen at -80C. Then, Liver was homogenated by gentleMACS Dissociators. Nuclei were isolated and protein-DNA complexes were incubated with antibodies against two kinds of E4BP4 and immunoprecipitated with IgG paramagnetic beads. Results: There were many significant peaks at the promoter/enhancer or intronic region of many genes. Conclusions: E4BP4 possibly regulates the expression of some genes linked to lipid metabolism in the liver.

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Expression profiles of colon macrophages from macrophage-specific E4BP4 transgenic mouse [ColonMac_RNA-seq]

Project description:Expression profiles of colon macrophages from macrophage-specific E4BP4 transgenic mouse [ColonMac_RNA-seq]

| PRJNA759127 | ENA

Gene expression profiling of colitis-associated and sporadic colorectal tumors in mice

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