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CREB ChIP-chip and HaloCHIP-chip experiments

ABSTRACT: Regulation of gene expression is essential for normal development and cellular growth. Transcriptional events are tightly controlled both spatially and temporally by specific DNA-protein interactions. In this study we finely map the genome-wide targets of the CREB protein across all known and predicted human promoters, and characterize the functional consequences of a subset of these binding events using high-throughput reporter assays. To measure CREB binding, we used HaloCHIP, an antibody-free alternative to the ChIP method that utilizes the HaloTag fusion protein, and also high-throughput promoter-luciferase reporter assays, which provide rapid and quantitative screening of promoters for transcriptional activation or repression in living cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE18347 | GEO | 2009/10/01

SECONDARY ACCESSION(S): PRJNA118157

REPOSITORIES: GEO

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CREB ChIP-chip and HaloCHIP-chip experiments

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ChIp-Chip using RNAP II, CREB C/EBPb and cJun antibody in undifferentiated or differentiated keratinocytes (expression)

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