Circulating microRNA expression profiling of Graves Disease
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE183576 | GEO | 2022/05/22
REPOSITORIES: GEO
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Project description:NGS was performed to detect human circRNAs. We found many upregulated and downregulated circRNAs in GD patients. Through high-throughput sequencing technology, 23215 upregulated and 13715 down-regulated circRNAs were detected between gd patient group and the healthy control group. Of the 218 circRNAs with significant difference, 359 were up-regulated and 309 were down-regulated.
2022-09-01 | GSE197637 | GEO
Project description:The trace element selenium (Se) plays an important role in thyroid hormone metabolism. Low Se intake is associated with atuoimmune hypothyroidism. The interaction of Se and Graves’ disease (GD) is only poorly characterized. We have investigated the importance of dietary Se supply for GD by performing a more in-depth analysis of a large cross-sectional study of 6152 participants from two counties within the Shaanxi Province, China, which are characterized by different habitual Se intakes, and studied the effects of different dietary Se supply (0.02, 0.18, 0.6 or 2.0 ppm Se) on disease development in a mouse model of GD. In the cross-sectional study, prevalence of GD in the two areas of different Se intake was similar, and the Se status of GD patients was not different from healthy control subjects. The dietary Se supply in the animal model was not affecting thyroid hormone levels, malondialdehyde concentrations, total antioxidant capacity or the titer of GD-causing TSH receptor autoantibodies (TRAb). Expression analysis of transcripts in spleen indicated regulatory effects of Se intake on genes implicated in the immune response, erythropoiesis and oxygen status. However, the humoral immune response including the ratio of CD4/CD8 or Th1/Th2 cells, and the concentration of Treg cells were similar between the experimental groups despite their varying Se intakes. Our data from both the epidemiological analysis and animal experiment do not indicate a major role of Se in the development of GD, and highlight that autoimmune hypothyroidism and GD differ in their relation to Se status.
2021-05-30 | GSE115152 | GEO
Project description:Hyperthyroidism is a kind of common autoimmune disease. It is widely accepted that B lymphocytes play a significant role in GD as they are the source of autoantibodies (TRAb) against the thyroid-stimulating hormone receptor (TSHR). We previously observed B cells infiltrated in the thyroid tissue from GD patients was significantly higher than that in healthy controls.In this study, our flow cytometry results indicated that the proportion of B cells in GD patients is much higher than that in control. To explore the underlying pathological function of B cells in GD, a microarray profiles was performed in these isolated B cells.
2019-09-02 | GSE136709 | GEO
Project description:As the fetal heart develops, cardiomyocyte proliferation potential decreases while fatty acid oxidative capacity increases, a highly regulated transition known as cardiac maturation. Small noncoding RNAs, such as microRNAs (miRNAs), contribute to the establishment and control of tissue-specific transcriptional programs. However, small RNA expression dynamics and genome wide miRNA regulatory networks controlling maturation of the human fetal heart remain poorly understood. Transcriptome profiling of small RNAs revealed the temporal expression patterns of miRNA, piRNA, circRNA, snoRNA, snRNA and tRNA in the developing human heart between 8 and 19 weeks of gestation. Our analysis revealed that miRNAs were the most dynamically expressed small RNA species throughout mid-gestation. Cross-referencing differentially expressed miRNAs and mRNAs predicted 6,200 mRNA targets, 2134 of which were upregulated and 4066 downregulated as gestation progresses. Moreover, we found that downregulated targets of upregulated miRNAs predominantly control cell cycle progression, while upregulated targets of downregulated miRNAs are linked to energy sensing and oxidative metabolism. Furthermore, integration of miRNA and mRNA profiles with proteomes and reporter metabolites revealed that proteins encoded in mRNA targets, and their associated metabolites, mediate fatty acid oxidation and are enriched as the heart develops.This study revealed the small RNAome of the maturing human fetal heart. Furthermore, our findings suggest that coordinated activation and repression of miRNA expression throughout mid-gestation is essential to establish a dynamic miRNA-mRNA-protein network that decreases cardiomyocyte proliferation potential while increasing the oxidative capacity of the maturing human fetal heart.
2024-01-26 | PXD044936 | Pride
Project description:NGS was performed to detect human miRNAs. We found many upregulated and downregulated miRNAs in HT patients. A total of 1310 dysregulated miRNAs were identified in PBMCs from HT patients and healthy volunteers, of which 659 were upregulated and 651 were Downregulated. Among them, a total of 655 miRNAs, 331 upregulated and 324 downregulated.
2020-06-30 | GSE148157 | GEO
Project description:In common with other autoimmune thyroid disorders (AITD), Gravesï¾? disease (GD) is a chronic autoimmune process. One salient feature of AITD is the inappropriate expression of HLA and other immune response molecules by thyroid follicular cells (TFC) this leading to postulate that stimuli generated in situ in the thyroid gland may contribute to trigger or perpetuate AITD. To identify the factors that determine chronicity, genes expression profiles of 2 normal and 6 autoimmune thyroid glands grouped according the course of disease (short course (SC) < 30 months, and long course (LC) > 30) were compared using Affymetrix Human Genome U133 Plus 2.0 Arrays followed by software aided pathway analysis and immunohistological studies. The results yielded over 200 differentially expressed (DE) genes pertaining to the immune system (Gene Ontology), of them nearly half of the DE genes were IFN-regulated. In all GD cases there was a predominance of genes of the humoral response and of antigen processing and presentation. While among chronicity genes, identified by a profile based algorithm, antigen presentation, viral response and chemokine genes ranked first.
2011-01-27 | E-MEXP-2612 | biostudies-arrayexpress
Project description:The goal of this study was comparative analysis of differentially expressed miRNA and their targets in human chondrosarcoma JJ012 not treated (Control) and JJ012 chondrosarcoma cell line treated with antitumorigenic neuropeptide PRP-1. The goal was to elucidate the major signal transduction pathways deregulation in sarcomagenesis. Total RNA extraction was followed by the analysis of RNA quality and integrity, Exiqon human miRNA panel of 743 unique miRNA assays,was performed. performed.211 unique gene targets were detected for downregulated miRNAs and 104 unique target genes for upregulated miRNAs with 28 target genes common for both. out of 122 upregulated and 109 downregulated , total 9 differentially statistically significant miRNAs(3 upregulated and 6 downregulated) fitting P<0.05 by Benjamini Hochberg were detected when comparing chondrosarcoma cell line treted to not treated control using human Exiqon arrays with biological triplicates.: miR192, regulating cell cycle checkpoinrts was upregulated in treated samples 4 fold, miR125b, known as tumor suppressor in sarcomas was upregulated 4.87 fold. miR20a was upregulated 5.29 , known to form complexes with Argonaute proteins-part of RNA induced silencing complex (RISC) Downregulated miRNas included miR509-3p (12.88 fold), its targets Yap, Rac1 and Yes1 known to be associated with Src kinase activity; miR589 was downregulated 7.46 fold , targets Src adaptors'. miR 302c was downregulated6.46 fold and miR199a=5p -5.66 fold , that acted as oncogenes in sarcomas. miR584 involved on cell migration and TGFbeta signaling was downregulated 4.78 fold Comparison of miRNA expression between control samples and samples treated with antiproliferative cytostatic mTORC1 inhibitor PRP-1 cytokine in human chondrosarcoma JJ012 cell line
2013-10-31 | E-GEOD-49545 | biostudies-arrayexpress
Project description:Increasing evidence suggests that CRS is a heterogeneous group of sinus disorders involving overlapping but distinct disease entities.The factors leading to different CRS phenotypes remain enigmatic. The role of miRNAs in the regulation of immunological and inflammatory processes is beginning to emerge.Thus, we examined microRNAs expression profiles in eosinophilic CRSwNP adn CRSsNP. Compared with controls, 31 differentially expressed miRNAs (30 downregulated and 1 upregulated miRNAs) in eosinophilic CRSwNP and 4 differentially expressed miRNAs (2 downregulated and 2 upregulated miRNAs) in CRSsNP were indentified. Real time RT-PCR was subsequently performed to verify the miRNA microarray result. Examination of miRNAs expression in eosinophilic CRSwNP and CRSsNP
2011-12-08 | E-GEOD-32300 | biostudies-arrayexpress
Project description:The goal of this study was comparative analysis of differentially expressed miRNA and their targets in human chondrosarcoma JJ012 and chondrocytes C28 cell line (control) to elucidate the major signal transduction pathways deregulation in sarcomagenesis. Total RNA extraction was followed by the analysis of RNA quality and integrity, Exiqon human miRNA panel of 743 unique miRNA assays, Illumina microarray HT12 platform and qRT-PCR verification of targets were performed. 189 downregulated and 147 upregulated miRNAs were detected when comparing chondrosarcoma cell line to control C 28 chondrocytes cell line using human Exiqon arrays with biological triplicates. 3045 target genes in total were detected with 587 common and 2458 unique target genes between up and downregulated miRNAs.The upregulated miRNAs were linked to the promotion of aggressive phenotype and the majority of the downregulated miRNAs were oncosuppressors. Among upregulated targtes for upregulated miRNAS were the cluster of cancer testis antigen (CTA) genes, located on X chromosome the major signal transduction pathways leading to sarcomagenesis. Comparison in triplicates the miRNA expression profile with Exiqon between human JJ012 chondrosarcoma cell line and C28 control chondrocytes
2013-08-28 | E-GEOD-48396 | biostudies-arrayexpress
Project description:In Graves’ disease (GD), a combination of genetic, epigenetic and environmental factors causes an autoimmune response to the thyroid gland, characterized by lymphocytic infiltrations and autoantibodies targeting the thyroid stimulating hormone receptor (TSHR) and other thyroid antigens. To identify the epigenetic changes involved in GD, we performed a genome-wide analysis of DNA methylation and enrichment of H3K4me3 and H3K27ac histone marks in sorted CD4+ and CD8+ T cells. We found 365 and 3322 differentially methylated CpG sites in CD4+ and CD8+ T cells, respectively. Among the hypermethylated CpG sites, we specifically found enrichment of genes involved in T cell signaling (CD247, LCK, ZAP70, CD3D, CD3E, CD3G, CTLA4 and CD8A) and decreased expression of CD3 gene family members. The hypermethylation was accompanied with the active chromatin histone modifications as we found decreased signals of H3K4me3 and H3K27ac marks at several T cell signaling genes in ChIP-seq analysis. In addition, we found hypermethylation of the TSHR gene first intron, where several GD-associated polymorphisms are located. Our results demonstrate an involvement of dysregulated DNA methylation and histone modifications at T cell signaling genes in GD patients.
2015-11-06 | GSE71955 | GEO