Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of 3D-culture balding and non-balding dermal papilla cells

ABSTRACT: Androgenetic alopecia (AGA, male patterned baldness) is a prevalent hair loss condition in males that develops due to the influence of androgens and genetic predisposition. With the aim of elucidating genes involved in AGA pathogenesis, we cultured immortalised balding human dermal papilla cells (BAB) and immortalised non-balding human dermal papilla cells (BAN) in 3-dimensional culture format, and compared gene expression using RNA-seq between BAB and BAN cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE184866 | GEO | 2022/09/27

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA-seq of 2D-culture balding and non-balding dermal papilla cells

Project description:Androgenetic alopecia (AGA, male patterned baldness) is a prevalent hair loss condition in males that develops due to the influence of androgens and genetic predisposition. With the aim of elucidating genes involved in AGA pathogenesis, we cultured immortalised balding human dermal papilla cells (BAB) and immortalised non-balding human dermal papilla cells (BAN) in 2-dimensional culture format, and compared gene expression using RNA-seq between BAB and BAN cells.

2022-09-27 | GSE184501 | GEO

Transcriptome analysis reveals differences in vasculature signalling between human dermal papilla cells from balding and non-balding scalp

Project description:Transcriptome analysis of hTERT-immortalized balding (BAB) and non-balding (BAN) dermal papilla cells derived from frontal and occipital scalp of male patients with androgenetic alopecia Hamilton grade IV. Interrogation of transcriptome differences between BAB and BAN after dihydrotestosterone (DHT, active metabolite of androgen) treatment revealed significant enrichment of vasculature-related genes among down-regulated genes in BAB compared to BAN.

2016-04-20 | GSE66664 | GEO

Dermal papilla signature gene expression in immortalized human dermal papilla cells from balding and non-balding scalp

Project description:Transcriptome analysis of DP signature gene expression in hTERT-immortalized balding (BAB) and non-balding (BAN) dermal papilla cells derived from frontal and occipital scalp of male patients with androgenetic alopecia Hamilton grade IV.

2016-04-20 | GSE66663 | GEO

Transcriptome comparison of balding and non-balding dermal papilla aggregates from NHEK co-culturing

Project description:Androgenetic alopecia (AGA, male patterned baldness) is a prevalent hair loss condition in males that develops due to the influence of androgens and genetic predisposition. The keratinocytes-surrounded spheroid dermal papilla (DP) at the base of the hair follicle is essential in hair morphogenesis and cycling. With the aim of elucidating genes involved in AGA pathogenesis, we co-cultured immortalised balding and non-balding human DP cells (DPC) derived from male AGA patients with epidermal keratinocyte (NHEK) using multi-interfacial polyelectrolyte complexation (MIPC) technique, and compared gene expression using RNA-seq between isolated balding and non-balding DP aggregates.

2021-06-23 | GSE93766 | GEO

Spatial transcriptome reveals disturbance of peri-infundibular immune infiltration in androgenetic alopecia

Project description:Several studies have suggested that in addition to androgen-sensitive dermal papilla cells, peri-infundibular immune infiltration is associated with the balding process in androgenetic alopecia (AGA). Nevertheless, investigations into the molecular signatures of this compartments are limited because none of the current techniques enable high-throughput profiling of specific pathological areas.

2023-08-11 | GSE240637 | GEO

RNA-seq of 2D-culture balding and non-balding dermal papilla cells

Project description:RNA-seq of 2D-culture balding and non-balding dermal papilla cells

| PRJNA764846 | ENA

RNA-seq of 3D-culture balding and non-balding dermal papilla cells

Project description:RNA-seq of 3D-culture balding and non-balding dermal papilla cells

| PRJNA766679 | ENA

Prostaglandin D2 Inhibits Hair Growth and Is Elevated in Bald Scalp of Men with Androgenetic Alopecia

Project description:Testosterone is necessary for the development of male pattern baldness, known as androgenetic alopecia (AGA); yet the mechanisms for decreased hair growth in this disorder are unclear. Here, we show that prostaglandin D2 synthase (PTGDS) is elevated at the mRNA and protein levels in bald scalp compared to haired scalp of men with AGA. The product of PTGDS enzyme activity, prostaglandin D2 (PGD2), is similarly elevated in bald scalp. During normal follicle cycling in mice Ptgds and PGD2 levels increase immediately preceding the regression phase, suggesting an inhibitory effect on hair growth. We show that PGD2 inhibits hair growth in explanted human hair follicles and when applied topically to mice. Hair growth inhibition requires the PGD2 receptor G protein-coupled receptor 44 (GPR44), but not the prostaglandin D2 receptor 1(PTGDR). Furthermore, we find that a transgenic mouse, K14-Ptgs2, which targets prostaglandin-endoperoxide synthase 2 expression to the skin, demonstrates elevated levels of PGD2 in the skin and develops alopecia, follicular miniaturization and sebaceous gland hyperplasia, which are all hallmarks of human AGA. These results define PGD2 as an inhibitor of hair growth in AGA and suggest the PGD2-GPR44 pathway as a potential target for treatment. 5 individuals with Androgenetic Alopecia were biopsied at both their haired and bald scalp for mRNA purification and microarray (total 10 arrays)

2012-03-26 | E-GEOD-36169 | biostudies-arrayexpress

Homo sapiens

Project description:Dermal papilla signature gene expression in immortalized human dermal papilla cells from balding and non-balding scalp

| PRJNA277690 | ENA

Transcripotome analysis of different locations of hair follicles (bulb, bulge) in androgenetic alopecia

Project description:To fully interrogate location-specific variations of the hair follicle in AGA and normal persons, we sought to perform a thorough and comprehensive transcripotome profiling of different locations of hair follicles (bulb portions, bulge portions) in balding and non-balding areas of AGA, with normal comparisons.

2017-12-25 | GSE101451 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data