Transcriptomics

Dataset Information

0

Morphological diversification and functional maturation of human astrocytes in glia-enriched cortical organoids transplanted in the mouse brain


ABSTRACT: Astrocytes, the most abundant glial cell type in the brain, are underrepresented in traditional cortical organoid models due to the delayed onset of cortical gliogenesis. Here, we introduce a novel glia-enriched cortical organoid model that exhibits accelerated astrogliogenesis. We demonstrated that induction of a gliogenic switch in a subset of progenitors enabled rapid derivation of astroglial cells, which account for 25-31% of the cell population within eight to ten weeks of differentiation. Intracerebral transplantation of these organoids reliably generated a diverse repertoire of cortical neurons and anatomical subclasses of human astrocytes. Spatial transcriptome profiling identified layer-specific expression patterns among distinct subclasses of astrocytes within the organoid transplants. Using an in vivo acute neuroinflammation model, we identified a subpopulation of astrocytes that rapidly activates proinflammatory pathways upon cytokine stimulation. Additionally, we demonstrated that CD38 signaling plays a crucial role in mediating metabolic and mitochondrial stress in reactive astrocytes. This model provides a robust platform for investigating human astrocyte function.

ORGANISM(S): Homo sapiens

PROVIDER: GSE185472 | GEO | 2024/01/16

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-08-21 | GSE275004 | GEO
2024-08-23 | GSE274547 | GEO
2024-09-03 | GSE276209 | GEO
2023-07-10 | GSE236429 | GEO
2023-06-13 | GSE224784 | GEO
2022-12-01 | GSE217511 | GEO
2011-10-19 | E-GEOD-33091 | biostudies-arrayexpress
2014-06-19 | E-GEOD-58614 | biostudies-arrayexpress
2011-10-20 | GSE33091 | GEO
2014-06-19 | GSE58614 | GEO