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Targeted Manipulation of Cellular RNA m6A Methylation at Single Base Level Using an Engineered dCas13b-METTL14 Fusion Protein

ABSTRACT: We engineered m6A methyltransferase with dCas13-M14 to achieve site-specific editing of m6A. The resultant m6A editors can be programmed with a guide RNA, allowing functional comparison of single site methylation in different mRNA regions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE185755 | GEO | 2024/10/12

REPOSITORIES: GEO

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Programmable RNA N6-methyladenosine editing by CRISPR-Cas9 conjugates

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Targeted Manipulation of Cellular RNA m6A Methylation at Single Base Level Using an Engineered dCas13b-METTL14 Fusion Protein

Project description:Targeted Manipulation of Cellular RNA m6A Methylation at Single Base Level Using an Engineered dCas13b-METTL14 Fusion Protein

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